Multi-dimensional oscillatory activity of mouse GnRH neurons in vivo
- Department of Physiology, Development and Neuroscience, Downing site, University of Cambridge, United Kingdom
- Zurich Center for Neuroeconomics, Department of Economics, University of Zurich, Switzerland
Figures
Figure 1
GCaMP expression in gonadotropin-releasing hormone (GnRH) neurons.
(A) Photomicrographs at the level of organum vasculosm of the laminae terminalis (OVLT) showing GFP immunofluorescence representing GCaMP6 (green), GnRH (purple), and merged channels showing double …
Figure 2
Gonadotropin-releasing hormone (GnRH) neuron dendron activity in male mice.
(A) A representative example of 24 hr GCaMP6 photometry recording showing abrupt dendron synchronized episodes (dSEs) occurring on lower amplitude baseline activity in a male mouse. Below, an …
Figure 3
Gonadotropin-releasing hormone (GnRH) neuron dendron activity in female mice.
(A) Representative example of 6 hr GCaMP6 photometry recordings from a female mice in (i) metestrus, (ii) diestrus, (iii) proestrus, and (iv) estrus. To the right of each trace are histograms …
Figure 4
Baseline high-frequency cluster activity of gonadotropin-releasing hormone (GnRH) neuron dendrons in male and female mice.
Representative 4 hr GCaMP recordings in (A) two male mice and (B) a female mouse across the different stages of the estrous cycle showing high-frequency baseline activity. The large increases in …
Figure 5
Slow oscillating increases in calcium activity on the afternoon of proestrus in female mice.
(A) Examples of 21 h GCaMP recordings from four proestrous female mice. Note the prolonged (>10 hr) gradual oscillating increase in calcium signal beginning on the afternoon of proestrus. The sharp …
Figure 6
Deconvolution of the GCaMP signal across the proestrous surge reveals multimodal activity patterns of gonadotropin-releasing hormone (GnRH) neuron dendron.
(A–B) Representative examples of 24 hr photometry recordings from two female mice starting at proestrus showing (i) the original recording, (ii) a 30 min rolling average highlighting the luteinizing …
