Pharyngeal neuronal mechanisms governing sour taste perception in Drosophila melanogaster
- Department of Bio & Fermentation Convergence Technology, Kookmin University, Republic of Korea
Figures
Figure 1 with 1 supplement
Ionotropic receptors are essential for sensing carboxylic acids in Drosophila melanogaster.
Screening of candidate 33 Ir mutants through binary food choice assay in presence of (A) 1% lactic acid (LA), (B) 1% citric acid (CA), and (C) 1% glycolic acid (GA) (n = 6–8 biological replicates). …
Figure 1—figure supplement 1
Structural analysis of (A) Ir94a1 and (B) Ir94h1 genes, (C) gender-dependent response to 1% carboxylic acid.
Figure 2 with 1 supplement
IR51b, IR94a, and IR94h are required to sense 1% lactic acid (LA), citric acid (CA), and glycolic acid (GA).
(A) Two-way choice assay of control and Ir51b2 (in-frame knock-in of GAL4) in 1% LA, CA, and GA (n = 6 biological replicates). (B) Rescue of Ir51b1 mutant defect to sense the taste of carboxylic …
Figure 2—figure supplement 1
Carboxylic acid profiling in the (A) presence or (B) absence of varying sucrose concentrations and (C) pH levels.
The control flies were w1118. All error bars represent SEMs. Single-factor ANOVA with Scheffe’s analysis was used as a post hoc test to compare multiple sets of data. Black asterisks indicate …
Figure 3 with 1 supplement
Ir51b, Ir94a, and Ir94h are expressed in pharynx of Drosophila melanogaster.
(A) Schematic diagrammatic representation of head of Drosophila depicting the location of Ir94a and Ir94h pharyngeal neuron. Images showing the expression of Ir94a and Ir94h neuron in different …
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Figure 3—source data 1
Original gel picture to Figure 3, panel E.
Given gel is the picture of RT-PCR resulting Ir51b expression in whole body, labellum, and pharynx.
- https://cdn.elifesciences.org/articles/101439/elife-101439-fig3-data1-v1.zip
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Figure 3—source data 2
Original gel picture to Figure 3, panel F.
Given gel is the picture of results from RT-PCR showing Ir51b expression in control (w1118) and poxn null alleles (poxn70 and poxnΔM22−B5) from pharynx.
- https://cdn.elifesciences.org/articles/101439/elife-101439-fig3-data2-v1.zip
Figure 3—figure supplement 1
Limited expression of Ir94a and Ir94h in (A-C) gustatory organs and (D) brain suboesophageal region.
Figure 4
Pharyngeal gustatory receptor neurons (GRNs) have a role in sensing the taste of carboxylic acids.
(A) Proboscis extension response (PER) assay, (B) Binary food choice assay of control and poxn−/− mutant (poxn70/poxn∆M2−-B5) in presence of 1% citric acid (CA) and 1% glycolic acid (GA) (n = 6 …
Figure 5 with 1 supplement
Stimulation of pharyngeal Ir94a and Ir94h gustatory receptor neurons (GRNs) using the optogenetic method.
(A) Schematic representation of optogenetics methods performed in the study (details in the Method section). (B) Optogenetic activation of Gr5a, Gr66a, Ir94a, and Ir94h GRNs by expressing …
Figure 5—figure supplement 1
Stimulation of pharyngeal Ir94a and Ir94h gustatory receptor neurons (GRNs) via chemogenetic method.
The control flies were w1118. All error bars represent SEMs. Single-factor ANOVA with Scheffe’s analysis was used as a post hoc test to compare multiple sets of data. Black colored circular dots …
Figure 6 with 2 supplements
Calcium imaging analysis showing the activation of pharyngeal Ir94a and Ir94h gustatory receptor neurons (GRNs) with carboxylic acid applied as stimulus.
(A) Heatmap analysis of pre- and post-stimulus with Ir94a-GAL4 flies with expression of GCaMP6s in control, mutant (Ir94a1 and Ir51b1) background, and rescue flies. (B) Quantification of peak …
Figure 6—figure supplement 1
Feeding assay analysis of control and mutants (A-B) .
The control flies were w1118. All error bars represent SEMs. Single-factor ANOVA with Scheffe’s analysis was used as a post hoc test to compare multiple sets of data. Different colored circular dots …
Figure 6—figure supplement 2
Recapitulation of sour receptors.
Videos
Video 1
Ex vivo calcium imaging of Ir94a-GAL4 from ventral cibarial sense organ (VCSO) in pharynx with the stimulus 1% citric acid (CA).
Video 2
Ex vivo calcium imaging of Ir94h-GAL4 from labral sense organ (LSO) in pharynx with the stimulus 1% glycolic acid (GA).
Additional files
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Supplementary file 1
Statistics for the data shown in Figure 2—figure supplement 1A.
- https://cdn.elifesciences.org/articles/101439/elife-101439-supp1-v1.docx
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Supplementary file 2
Statistics for the data shown in Figure 2—figure supplement 1B.
- https://cdn.elifesciences.org/articles/101439/elife-101439-supp2-v1.docx
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Supplementary file 3
Statistics for the data shown in Figure 2—figure supplement 1C.
- https://cdn.elifesciences.org/articles/101439/elife-101439-supp3-v1.docx
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MDAR checklist
- https://cdn.elifesciences.org/articles/101439/elife-101439-mdarchecklist1-v1.pdf
