(A) The illustration of the side-view observation device. (B) Exploded view diagram of the side-view observation device. (C) The definition of the bottom- and the side-view of a spheroid sample. (D) …
(A) Light path of the bottom-view observation of the spheroid in the agarose micro-well. (B) Light path of side-view observing of the spheroid in the agarose micro-well. (C) The absorption spectrum …
(A) Time-lapse images of DU 145 spheroid formation in an agarose well (1 × 104 cells/well, scale bar = 100 μm). (B) Changes in height and width during the growth process of the spheroids, as well as …
(A) The bottom-view images of the spheroid in the petri dish, cells extrude from the spheroid, forming sprouts. (B) Statistics on the changes in total width, inner width, and migration distance of …
(A) Time-lapse bottom- and side-view images of DU 145 spheroids formed from different initial cell concentrations (2.5 × 103, 5 × 103, 1 × 104, and 2 × 104 cells/well). Blue arrows indicate the …
(A) Time-lapse bottom- and side-view images of fusion body formed by two 7-day-old DU 145 cell spheroids with ideal ball-like structure (i) and irregular cell spheroid (ii). Red arrows point to the …
(A) Time-lapse bottom- and side-view images of 3- and 7-day-age DU 145 cell spheroids (1 × 104 cells/spheroid) under DOX (50 μg/mL) treatment. (B) The changes of DU 145 spheroids’ width and height …
(A) The top- and side-view images of the side-view observation petri dish/device. (B) The magnets inside the device.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Cell line (human prostate cancer cell) | DU 145 | Pricella Biotechnology | CL-0075 (RRID:CVCL_0105) | |
Cell line (human natural killer cell) | NK-92 | Pricella Biotechnology | CL-0530 (RRID:CVCL_2142) | |
Chemical compound, drug | DMEM culture medium | Gibco | C11995500BT | |
Chemical compound, drug | Fetal bovine serum | Bio-channel | BC-SE-FBS01 | |
Chemical compound, drug | Penicillin–streptomycin solution | Beyotime Biotechnology | C0222 | |
Chemical compound, drug | NK-92 special medium | Pricella Biotechnology | CM-0530 | |
Chemical compound, drug | Agarose | Aladdin Scientific | 11966311 | |
Chemical compound, drug | Phosphate-buffered saline | Beijing DingGuo ChangSheng Biotechnology Co Ltd | BF-0011 | |
Chemical compound, drug | Ultrapure water | ELGA Corporation | PURELAB flex system | |
Software, algorithm | Origin | OriginLab Corporation | 2021 (RRID:SCR_014212) | |
Software, algorithm | ImageJ | LOCI, University of Wisconsin | (RRID:RCR_003070) | |
Other | Xenon lamp | Beijing zhongjiao Jinyuan Technology Co, Ltd | CEL-TCX250 | Light source |
Other | Fiber optic spectrometer | Fuxiang Optics Co, Ltd | PG2000-Pro-EX | Instrument |
Other | Microscope | Nikon | ECLIPSE Ti (RRID:SCR_021242) | Instrument |
Other | D-LH/LC lamphouse | Nikon | Light source | |
Other | 3D printer | Bambu Lab | P1P | Instrument |