Valence and salience encoding in the central amygdala
- Department of Psychiatry and Behavioral Sciences, University of Washington, United States
- Department of Statistics, University of Washington, United States
- Department of Biostatistics, University of Washington, United States
- Department of Pharmacology, University of Washington, United States
Figures
Figure 1 with 1 supplement
Acquisition of calcium signals during Pavlovian appetitive and fear conditioning.
(A) CeA-GABAergic neurons were labelled with GCaMP6m, and their activity was recorded using a miniature microscope (Inscopix) via an implanted GRIN lens (left). A representative image of GCaMP6m …
Figure 1—figure supplement 1
GRN lens placements.
Histological reconstructions of GRIN lens locations.
Figure 2
Determination of CeA responses to appetitive and fearful stimuli.
(A) Left: Example traces of CeA neurons that were responsive (upper three traces) and not responsive to food reward (bottom three traces). Right: Example traces of CeA neurons that were responsive …
Figure 3 with 1 supplement
Responses of CeA neurons during Pavlovian appetitive conditioning.
(A) Top: Heat maps of CeA neurons aligned to CSFood onset (top left, 1078 neurons) and head entry (top right) from day 1 of Pavlovian appetitive conditioning. Bottom: Heat maps of CeA neurons …
Figure 3—figure supplement 1
CSFood and food response in the CeA neurons.
(A) Proportion of CSFood-only, Food-only, CSFood+ Food, and not significant neurons during appetitive learning. Blue and orange in the outer pie chart indicate the number of excited and inhibited …
Figure 4 with 1 supplement
Responses of CeA neurons during Pavlovian fear conditioning.
(A) Heat maps of CeA neurons aligned to CSShock onset (519 neurons) from day 1 of Pavlovian fear conditioning. Solid orange lines indicate CSShock, and a dotted red line represents shock delivery. (B…
Figure 4—figure supplement 1
CSShock and shock response in the CeA neurons.
(A) Proportion of CSShock-only, Shock-only, CSShock +Shock, and not significant neurons during fear conditioning. Blue and orange in the outer pie chart indicate the number of excited and inhibited …
Figure 5 with 3 supplements
Salience and valence encoding in the CeA.
(A) Among 872 neurons from appetitive conditioning and 519 neurons from fear conditioning, 303 neurons were registered during both learning. Heat maps of these 303 neurons during –10 s to 25 s after …
Figure 5—figure supplement 1
Characteristics of CS and US responses in the CeA.
(A) Peak time comparisons of food-excited (blue) vs. shock-excited (red) neurons from food/shock delivery (t=21.76, P<0.0001). (B) Maximum Z-score comparisons of food-excited vs. shock-excited …
Figure 5—figure supplement 2
Determining responsive neurons using the “theoretical” distribution of the Wilcoxon rank sum test, which assumes independence between timepoints.
(A) Each trial’s pre vs. post-stimulus activity was compared using Wilcoxon rank sum test. Neurons showing significant activity to the stimulus in more than 7 trials (appetitive; food or CSFood) or …
Figure 5—figure supplement 3
The order of valence conditioning did not affect the encoding of food, shock, and CSs in the CeA.
(A–D) Data of 165 neurons from appetitive → fear learning order. (A) The averaged Z-scored activity during –10 s to 25 s after CSFood onset from appetitive learning (left) and during –10 s to 25 s …
Author response image 1
Videos
Video 1
CeA activity during the appetitive conditioning day 10.
Video 2
CeA activity during the fear conditioning.
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-GFP (chicken polyclonal) | abcam | RRID:AB_300798 | (1:6000) |
| Antibody | Anti-chicken- AlexaFluor 488 (donkey polyclonal) | Jackson ImmunoResearch | RRID:AB_2340375 | (1:250) |
| Strain, strain background | AAV1-FLEX-GCaMP6m | University of Washington | N/A | |
| Strain, strain background (Mus musculus) | B6J.129S6(FVB)-Slc32a1tm2(cre)Lowl/M warJ | Jackson laboratory | RRID:IMSR_JAX:028862 | 4 males and 6 females |
| Software, algorithms | MATLAB | The MathWorks, Inc | RRID:SCR_001622 | |
| Software, algorithms | R | The R Foundation | RRID:SCR_001905 | |
| Software, algorithms | Med-PC | Med Associates, Inc | RRID:SCR_012156 | |
| Software, algorithms | Graph Pad Software | GraphPad Software, Inc | RRID:SCR_002798 | |
| Software, algorithms | Inscopix data processing software | Inscopix | ||
| Software, algorithms | Inscopix data acquisition software | Inscopix | IDAS 1.5.4 | |
| Other | DAPI Fluoromount-G | SouthernBiotech | 0100–20 | DAPI staining |
| Other | Gradient-index (GRIN) lens | Inscopix | 1050–004413 | ProViewTM Integrated Lens 0.6mm x7.3mm |
| Other | Dental cement | Lang Dental | 1530BLK | Contemporary Ortho-Jet Powder BLACK Powder |
| Other | Dental cement | Lang Dental | 1504BLK | Contemporary Ortho-Jet Liquid |
| Other | Anchoring screws | Antrin Miniature Specialties, Inc | AMS 120/1 P-25 | Screws for anchoring a lens |
Additional files
-
MDAR checklist
- https://cdn.elifesciences.org/articles/101980/elife-101980-mdarchecklist1-v1.docx
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Supplementary file 1
Results of statistical analyses.
- https://cdn.elifesciences.org/articles/101980/elife-101980-supp1-v1.docx
