Distinct trafficking routes of polarized and non-polarized membrane cargoes in Aspergillus nidulans
- National and Kapodistrian University of Athens, Greece
- Institute of Molecular Biology and Biotechnology, Greece
Abstract
Membrane proteins are sorted to the plasma membrane (PM) via Golgi-dependent trafficking. However, our recent studies challenged the essentiality of Golgi in the biogenesis of specific transporters. Here, we investigate the trafficking mechanisms of membrane proteins by following the localization of the polarized R-SNARE SynA versus the non-polarized transporter UapA, synchronously co-expressed in wild-type or isogenic genetic backgrounds repressible for conventional cargo secretion. In wild-type, the two cargoes dynamically label distinct secretory compartments, highlighted by the finding that, unlike SynA, UapA does not colocalize with the late-Golgi. In line with early partitioning into distinct secretory carriers, the two cargoes collapse in distinct ERES in a sec31ts background. Trafficking via distinct cargo-specific carriers is further supported by showing that repression of proteins essential for conventional cargo secretion does not affect UapA trafficking, while blocking SynA secretion. Overall, this work establishes the existence of distinct, cargo-dependent, trafficking mechanisms, initiating at ERES and being differently dependent on Golgi and SNARE interactions.
Data availability
Strains and plasmids are available upon request. The authors affirm that all data necessary for confirming the conclusions of the article are present within the article, figures, tables and source data files.
Article and author information
Author details
Georgia Maria Sagia
Funding
Hellenic Foundation for Research and Innovation (3112/KE18458)
- George Diallinas
Hellenic Foundation for Research and Innovation (19067)
- George Diallinas
Fondation Sante (scholarship)
- Xenia Georgiou
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2024, Sagia et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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Distinct trafficking routes of polarized and non-polarized membrane cargoes in Aspergillus nidulans
eLife 13:e103355.
https://doi.org/10.7554/eLife.103355
