CadN405 R7 cells were generated with MARCM, using GMR-FLP and positively labeled with CD4-tdGFP. Fast (1 min interval) time-lapse imaging was performed at P + 28%. (a), The average numbers of filopodia per growth cone are not significantly different between wt and CadN405. (b) Mutant filopodia are slower, (for transient, means wt: 1.303 (n = 143), CadN405: 0.791 (n = 169), p<0.0001; for stable, means wt: 0.898 (n = 10), CadN405: 0.636 (n = 5) p = 0.0199) and (c) shorter (for transient, means wt: 1.542 (n = 143), CadN405: 0.939 (n = 169), p <0.0001; for stable: means wt: 3.707 (n = 10), CadN405: 2.275 (n = 5), p = 0.1257). (d) CadN405 R7 growth cones at the correct layer. Scale bars, 5 μm.