Gustatory-mediated avoidance of bacterial lipopolysaccharides via TRPA1 activation in Drosophila
- KU Leuven, Belgium
- VIB, Belgium
- University of Leuven School of Medicine, Belgium
- Neuroelectronics Research Flanders, Belgium
- NTNU, Norway
- Hôpital Pitié-Salpétrière, France
- Université Pierre et Marie Curie, Sorbonne Universités, France
Figures
Figure 1 with 1 supplement
Gustatory dTrpA1-dependent avoidance of LPS in Drosophila melanogaster.
(A,C) Preference index (PI) of control CS10w1118 (A) and dTrpA1-deficient (C) male flies for control food over LPS-supplemented food. PI was calculated for the consumption of the control solution …
Figure 1—figure supplement 1
dTrpA1-dependent aversion to LPS in Drosophila.
(A) Left, microtiter plate used for the binary food choice test. Right after feeding, flies were visually inspected for abdomen color. (B) Female control CS10w1118 flies avoid food supplemented with …
Figure 2
dTrpA1 expression in gustatory neurons is required for avoidance of LPS during oviposition.
(A) Preference index for oviposition in control food of wild type versus dTrpA1-/- flies (n ≥ 5). (B) Oviposition preference of Gr66a>TNT flies (n ≥ 6). (C) Oviposition preference of Gr66a>dTrpA1 …
Figure 3 with 2 supplements
dTrpA1 is expressed in a subset of Gr66a-expressing neurons that can be directly stimulated by LPS.
(A) Immunofluorescence analysis of Gr66a-IRES-GFP,UAS-RFP;dTrpA1Gal4 adult proboscis. Anti-GFP immunohistochemistry (in green in the Merge panel) labels taste neurons while anti-RFP (in red in the …
Figure 3—figure supplement 1
dTRPA1 is not expressed in tarsal Gr66a-expressing neurons.
Immunofluorescence analysis of Gr66a-IRES-GFP,UAS-RFP;dTrpA1Gal4 fly legs. Anti-GFP immunohistochemistry labels taste neurons (in Green in the Merge panel) while anti-RFP labels dTrpA1-expressing …
Figure 3—figure supplement 2
LPS does not alter the proboscis extension reflex.
Proboscis extension reflex (PER) to 100 mM sucrose alone or mixed with 1 mg/ml LPS. The sucrose has been offered both before and after LPS. The graphs show the frequency of extension as mean of 19 …
Figure 4 with 4 supplements
LPS stimulates dTRPA1.
(A) Intracellular Ca2+ imaging in cultured brain L3 neurons expressing RFP and GCaMP5 under the control of dTrpA1Gal4 in baseline conditions and during extracellular perfusion with LPS (30 µg/ml), …
Figure 4—figure supplement 1
LPS stimulates dTRPA1 in vivo.
(A) Examples of intracellular Ca2+ imaging recordings performed in the ventral nerve cord of Drosophila larvae in an in vivo larval fillet preparation. The arrows indicate the time of application of …
Figure 4—figure supplement 2
Images of a culture of cells from third instar larvae brains.
Left, bright field; center, immuno-fluorescence staining with the neuronal marker Elav; right, merged images.
Figure 4—figure supplement 3
LPS stimulates Drosophila neurons in vitro in a dTRPA1-dependent manner.
(A) Examples of the effects of extracellular application of LPS (60 µg/ml), AITC (100 µM) or a high K+ solution on the intracellular Ca2+ concentration recorded in neurons isolated from control (dTrp…
Figure 4—figure supplement 4
LPS-induced responses in HEK293T cells transfected with dTRPA1 channels.
(A and B) Intracellular Ca2+ imaging experiment showing that E. coli LPS (60 µg/ml) stimulates cells transfected with dTRPA1-A (A) or dTRPA1-B (B) (responsive to 100 µM AITC, black traces), but not …
