(a) Growth in rat brain size from E18, P0, P4, P8, P12, P16, P20 to adult. Brains are overlaid on a 1 cm x 1 cm grid. (b) Mean weight (in grams) of E18 (n=3), P0 (n=6), P4 (n=5), P8 (n=5), P12 (n=5), P16 (n=5), P20 (n=5), P24 (n=6) and in adult (n=9) rat brains. Error bars indicate SD. (c) Parasagittal section double stained for calbindin-immunoreactivity (green) and Purkinje cell protein 4 immunoreactivity (pcp4; red), illustrating the superficial layers of the medial entorhinal cortex and parasubiculum. Calbindin+ neurons (green) are in layer 2, pcp4+ neurons (red) are in layer 3 MEC. (d) Development of mean layer width (in μm) of layer 1 (light-blue), layer 2 (green), layer 3 (red), layer 4 (gray-blue) and layer 5/6 (purple) from P0 to P24 and in adult rat medial entorhinal cortex. Scale bars 250 µm. PaS- Parasubiculum; L1- Layer 1; L2- Layer 2; L3- Layer 3; D- Dorsal; V-Ventral.