1. Neuroscience

Modulation of anxiety and fear via distinct intrahippocampal circuits

  1. Elif Engin  Is a corresponding author
  2. Kiersten S. Smith
  3. Yudong Gao
  4. David Nagy
  5. Rachel A. Foster
  6. Evgeny Tsvetkov
  7. Ruth Keist
  8. Florence Crestani
  9. Jean-Marc Fritschy
  10. Vadim Y. Bolshakov
  11. Mihaly Hajos
  12. Scott A. Heldt
  13. Uwe Rudolph
  1. McLean Hospital, United States
  2. Harvard Medical School, United States
  3. The University of Tennessee Health Science Center, United States
  4. Yale School of Medicine, United States
  5. Russian Academy of Sciences, Russia
  6. University of Zurich, Switzerland
https://doi.org/10.7554/eLife.14120
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Cite this article
  1. Elif Engin
  2. Kiersten S. Smith
  3. Yudong Gao
  4. David Nagy
  5. Rachel A. Foster
  6. Evgeny Tsvetkov
  7. Ruth Keist
  8. Florence Crestani
  9. Jean-Marc Fritschy
  10. Vadim Y. Bolshakov
  11. Mihaly Hajos
  12. Scott A. Heldt
  13. Uwe Rudolph
(2016)
Modulation of anxiety and fear via distinct intrahippocampal circuits
eLife 5:e14120.
https://doi.org/10.7554/eLife.14120
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4 figures and 4 tables

Figures

Figure 1 with 5 supplements
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Targeted reduction of α2 expression in CA1, CA3 or DG.

(A) Generation of the α2F/F control and α2CA1KO, α2CA3KO, α2DGKO mice. (B) Top: False color images showing the α2 staining intensity in immunohistochemically stained sections. Cooler colors = less staining. Bottom: Semi-quantitative comparisons of α2 staining. (C) α2 mRNA expression (see sample ROI’s on the right), expressed as% of α2F/F control.*p<0.05, **p<0.01, ***p<0.001 compared to corresponding α2F/F group.

https://doi.org/10.7554/eLife.14120.003
Figure 1—figure supplement 1
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Immunohistochemical localization of GABAAR α2 subunits in conditional knockout mice bred on a 129X1/SvJ background.

(A) False color images showing α2 staining intensity in immunohistochemically stained sections in α2F/F mice.Cooler colors = less staining. (B) Same as A for α2CA1KO, (C) Same as A for α2CA3KO, (D) Same as A for α2DGKO.

https://doi.org/10.7554/eLife.14120.004
Figure 1—figure supplement 2
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Expression of α1 and α5 subunits in conditional knockout mice.

(A) Top: Representative gray-scale images showing the α1 staining intensity in immunohistochemically stained sections from the hippocampus, cortex and the amygdala of α2F/F, α2CA1KO, α2CA3KO and α2DGKO mice. Bottom: Semi-quantitative comparisons of α1 staining intensity between α2F/F, α2CA1KO, α2CA3KO and α2DGKO mice in CA1, CA3, DG, cortex and the amygdala. (B) Same as A for α5.

https://doi.org/10.7554/eLife.14120.005
Figure 1—figure supplement 3
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Expression of α3 and α4 mRNA in conditional knockout mice.

(A) Gabra3 expression in CA1, CA3, DG, cortex and the amygdala of α2F/F, α2CA1KO, α2CA3KO and α2DGKO mice expressed as percentage of α2F/F control. (B) Same as A for Gabra4. *p<0.05 in comparison to the corresponding α2F/F control group.

https://doi.org/10.7554/eLife.14120.006
Figure 1—figure supplement 4
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Miniature inhibitory postsynaptic currents.

Mean ( ± S.E.M.) decay time, frequency, amplitude of mIPSCs recorded from (A) the CA1 pyramidal neurons in α2CA1KO and α2F/F sections, (B) the CA3 pyramidal neurons in α2CA3KO and α2F/F sections, (C) the DG granule cells in α2DGKO and α2F/F sections. **p<0.01, ***p<0.001. CTRL=vehicle-treated, DZP=diazepam-treated.

https://doi.org/10.7554/eLife.14120.007
Figure 1—figure supplement 5
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Tests of hippocampal function.

(A) Mean ( ± S.E.M.) percentage of time spent freezing during the tone 24 hr after auditory fear-conditioning using a delay (black) or trace (white) protocol.*p<0.05, ***p<0.001, different from the corresponding delay group. No significant differences were found in planned pair-wise comparisons of the genotypes within the trace-conditioned groups or within the delay-conditioned groups. (B) Mean ( ± S.E.M.) percentage of time spent freezing during re-exposure to the context 24 hr after contextual fear-conditioning. ***p<0.001, different from α2F/F. (C) Mean ( ± S.E.M.) distance to platform during probe trials. *p<0.05, ***p<0.001, different from α2F/F; gray: α2CA3KO, red: α2DGKO.

https://doi.org/10.7554/eLife.14120.008
Figure 2 with 1 supplement
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Behavioral tests of anxiety and locomotor activity.

(A) Left: Activity heat maps on the EPM of representative α2F/F, α2CA1KO, α2CA3KO and α2DGKO mice treated with vehicle or diazepam. Right: Percentage (Mean ± S.E.M.) of time spent in the open arms of the EPM. (B) Left: Activity heat maps on the LDB of representative α2F/F, α2CA1KO, α2CA3KO and α2DGKO mice treated with vehicle or diazepam. The dark (red) and lit (yellow) compartments of the box are outlined for ease of visualization. Right: Percentage (Mean ± S.E.M.) of time spent in the lit compartment of the LDB. (C) Mean ( ± S.E.M.) distance travelled in the open field.

https://doi.org/10.7554/eLife.14120.010
Figure 2—source data 1

Raw data for elevated plus maze and light/dark box figures.

https://doi.org/10.7554/eLife.14120.011
Figure 2—figure supplement 1
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Additional measures in tests of anxiety-like behavior.

(A) Left: Percentage of entries into the open arms in elevated plus maze in F/F control, α2CA1KO, α2CA3KO and α2DGKO mice bred on the C57BL/6J background. Right: Total distance travelled in the elevated plus maze. (B) Left: Percentage of time spent in the open arms, Right: Percentage of entries into the open arms in F/F control, α2CA1KO, α2CA3KO and α2DGKO mice bred on the 129x1/SvJ background. (C) Number of entries into the light compartment in light/dark box in F/F control, α2CA1KO, α2CA3KO and α2DGKO mice bred on the 129×1/SvJ background. +p<0.06, *p<0.05, **p<0.01 in comparison to the corresponding vehicle group.

https://doi.org/10.7554/eLife.14120.012
Figure 3 with 1 supplement
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Behavioral tests of fear.

(A) Representative recordings of “Startle Stimulus Only” and “Tone + Startle Stimulus” trials in a α2F/F control mouse treated with vehicle. The increased startle amplitude in “Tone + Startle” trials represents fear-potentiation of the startle response. (B-E) Mean ( ± S.E.M.) startle amplitude in “No Tone” and “Tone” startle trials in vehicle and diazepam-treated (B) α2F/F, (C) αCA1KO, (D) α2CA3KO, (E) α2DGKO mice. (F) Mean ( ± S.E.M.) percent FPS in trials preceded by the tone. Asterisks represent significant difference from the corresponding vehicle group. (G) Mean ( ± S.E.M.) number of licks recorded in the pretest session of the VCT where drinking is not punished (This session does not involve drug administration). (H) Mean ( ± S.E.M.) number of licks recorded in the test session where every 20th lick is punished in vehicle- or diazepam-treated mice. Asterisks represent significant difference from the corresponding vehicle group. *p<0.05, **p<0.01, ***p<0.001.

https://doi.org/10.7554/eLife.14120.014
Figure 3—source data 1

Raw data for fear-potentiated startle and Vogel conflict test figures.

https://doi.org/10.7554/eLife.14120.015
Figure 3—figure supplement 1
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Additional measures in tests of fear-related behavior.

(A-D) Mean (± S.E.M.) startle amplitude in response to different intensities of white noise bursts measured in arbitrary units based on the displacement of load cells during the first three days (Habituation sessions) of the FPS experiment in (A) α2F/F, (B) α2CA1KO, (C) α2CA3KO, (D) α2DGKO mice. (E) Mean ( ± S.E.M.) shock sensitivity measured by the average motion registered during 0.4 mA shocks. (F) Mean ( ± S.E.M.) number of unpunished licks on test day in vehicle- and diazepam-treated α2F/F control mice.

https://doi.org/10.7554/eLife.14120.016
Figure 4 with 1 supplement
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Evoked theta oscillations in the vHPC.

(A) Stimulation and recording sites and representative traces showing vHPC theta activity before and after diazepam injection in a α2F/F mouse. (B-E) Mean ( ± S.E.M.) peak frequency in the theta range at different stimulation intensities before (Baseline), and 30 min (30min post-DZP) and 60 min (60min post-DZP) following diazepam injection in (B) α2F/F, (C) α2CA1KO, (D) α2CA3KO, (E) α2DGKO mice. Asterisks represent significant difference from the baseline at the given stimulating current, with top ones for 30 min and the lower ones for 60 min post-injection. (F) Change from baseline slope in the stimulation intensity – peak frequency function 30 min (left) or 60 min following diazepam injection.#p<0.09, *p<0.05, **p<0.01, ***p<0.001 compared to corresponding α2F/F group.

https://doi.org/10.7554/eLife.14120.018
Figure 4—source data 1

Raw data for peak frequency and stimulation intensity – peak frequency slope figures.

https://doi.org/10.7554/eLife.14120.019
Figure 4—figure supplement 1
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Frequency and power of theta range oscillations.

A-D Mean ( ± S.E.M.) peak frequency in the theta range in dHPC recorded before, 30min after and 60min after diazepam administration in (A) α2F/F, (B) α2CA1KO, (C) α2CA3KO, (D) α2DGKO mice.**p<0.01, ***p<0.001 compared to pre-diazepam baseline at the given stimulating current. (E) Mean ( ± S.E.M.) change from baseline slope in the stimulation intensity – peak frequency function for dHPC recordings 30 min (left) or 60 min following diazepam injection,*p<0.05 compared to corresponding α2F/F group. (F) Mean ( ± S.E.M.) theta power in the vHPC normalized to baseline power 60 min following diazepam injection. (G) Same as F for dHPC recordings. *p<0.05 compared to corresponding α2F/F group.

https://doi.org/10.7554/eLife.14120.020

Tables

Table 1

Results of omnibus statistical tests of experiments for the general characterization of α2CA1KO, α2CA3KO and α2DGKO mice.

https://doi.org/10.7554/eLife.14120.009
1. Immunohistochemistry
One-Way ANOVA; Factor: Genotype
α2 Subunitα1 Subunitα5 Subunit
CA1F(3,10)=9.44P=0.003F(3,9)=0.08p=0.97F(3,9)=0.10p=0.96
CA3F(3,10)=5.05P=0.02F(3,9)=0.01p=0.99F(3,9)=0.13p=0.94
DGF(3,10)=9.08P=0.003F(3,9)=0.00p=1.00F(3,9)=0.06p=0.98
CortexF(3,10)=1.90p=0.19F(3,9)=0.01p=0.83F(3,9)=0.01p=0.99
AmygdalaF(3,10)=1.28p=0.34F(3,9)=0.21p=0.89F(3,9)=0.36p=0.78
2. Quantitative PCR
One-Way ANOVA; Factor: Genotype
α2 Subunitα3 Subunitα4 Subunit
CA1F(3,16)=10.66p<0.001F(3,16)=0.10p=0.96F(3,16)=2.48p=0.1
CA3F(3,16)=10.53p<0.001F(3,16)=0.67p=0.58F(3,16)=4.74p=0.02
DGF(3,16)=7.32p=0.003F(3,12)=1.96p=0.17F(3,12)=2.90p=0.08
CortexF(3,16)=15.69p<0.001
AmygdalaF(3,16)=3.01p=0.06
3. Slice Electrophysiology
Two-Way Mixed Factorial ANOVA; Factors: Genotype (between-subjects), Drug (within-subjects)
CA1AmplitudeFrequencyDecay Time
GenotypeF(1,38)=1.83p=0.18F(1,38)=2.80p=0.10F(1,38)=1.94p=0.17
DrugF(1,38)=1.49p=0.23F(1,38)=9.38p=0.004F(1,38)=107.96p<0.001
Genotype x Drug InteractionF(1,38)=2.91p=0.09F(1,38)=3.11p=0.08F(1,38)=0.82p=0.37
CA3AmplitudeFrequencyDecay Time
GenotypeF(1,21)=2.36p=0.14F(1,21)=1.85p=0.19F(1,21)=0.95p=0.34
DrugF(1,21)=0.66p=0.42F(1,21)=0.09p=0.77F(1,21)=30.54p<0.001
Genotype x Drug InteractionF(1,21)=0.21p=0.65F(1,21)=1.90p=0.18F(1,21)=0.25p=0.62
DGAmplitudeFrequencyDecay Time
GenotypeF(1,21)=1.50p=0.23F(1,21)=1.58p=0.22F(1,21)=0.01p=0.91
DrugF(1,21)=2.58p=0.12F(1,21)=1.58p=0.22F(1,21)=47.42p<0.001
Genotype x Drug InteractionF(1,21)=0.54p=0.47F(1,21)=1.33p=0.26F(1,21)=0.30p=0.59
4. Tests of Hippocampal Function
Delay – Trace Fear Conditioning
Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Condition (between-subjects)
% Freezing
GenotypeF(3,49)=0.38p=0.77
ConditionF(1,49)=41.57p<0.001
Genotype x Cond. InteractionF(3,49)=0.71p=0.55
Contextual Fear Conditioning
One-Way ANOVA; Factor: Genotype (between-subjects)
% Freezing
GenotypeF(3,35)=5.47p=0.003
Morris Water Maze
Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Day (within-subjects)
Time to platform
GenotypeF(3,80)=5.17p=0.01
DayF(5,80)=244.12p<0.001
Genotype x Day InteractionF(5,80)=1.36p=0.19
Table 2

Results of omnibus statistical tests of measured parameters in behavioral tests of anxiety and general locomotion.

https://doi.org/10.7554/eLife.14120.013
1. Elevated Plus Maze (C57BL/6J)
Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Drug (between-subjects)
% Open Arm Time% Open Arm EntriesDistance Travelled
GenotypeF(3,75)=1.41p=0.25F(3,75)=0.13p=0.94F(3,75)=0.85 F(3, 69) = 1.11p=0.47
DrugF(1,75)=16.48p<0.001F(3,75)=2.33p=0.13F(3,75)=0.61p=0.44
Genotype x Drug InteractionF(3,63)=1.54p=0.21F(3,75)=1.47p=0.23F(3,75)=1.13p=0.34
1’. Elevated Plus Maze (129X1/SvJ)
Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Drug (between-subjects)
% Open Arm Time% Open Arm EntriesDistance Travelled
GenotypeF(3,55)=0.17p=0.92F(3,55)=1.59p=0.20F(3,55)=1.17p=0.33
DrugF(1,55)=11.49P=0.001F(1,55)=3.4649p=0.07F(1,55)=1.28p=0.29
Genotype x Drug InteractionF(3,55)=2.28p=0.09F(3,55)=0.50p=0.69F(3,55)=0.82p=0.49
2. Light / Dark Box
Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Drug (between-subjects)
% Time in LightEntries to Light
GenotypeF(3,73)=5.03p=0.003F(3,73)=0.84p=0.48
Drug(F(1,73)=26.00p<0.001F(1,73)=1.45p=0.23
Genotype x Drug InteractionF(3,73)=5.53p=0.002F(3,73)=2.17p=0.09
3. Open Field
Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Drug (between-subjects)
Distance Travelled
GenotypeF(3,56)=1.78p=0.16
DrugF(1,56)=0.04p=0.84
Genotype x Drug InteractionF(3,56)=0.43p=0.73
Table 3

Results of omnibus statistical tests of measured parameters in behavioral tests of fear.

https://doi.org/10.7554/eLife.14120.017
1. Fear-Potentiated Startle
Within-Genotype Comparisons
Two-Way Factorial ANOVA; Factors: Tone/No Tone (within-subjects), Drug (between-subjects)
α2F/Fα2CA1KO
ToneF(1,28)=33.75p<0.001F(1,20)=49.17p<0.001
DrugF(1,28)=0.30p=0.59F(1,20)=0.51p=0.48
Tone x Drug InteractionF(1,28)=9.95p=0.004F(1,20)=0.02P=0.89
α2CA3KOα2DGKO
ToneF(1,18)=16.60p<0.001F(1,20)=54.46p<0.001
DrugF(1,18)=0.09p=0.77F(1,20)=0.73p=0.40
Tone x Drug InteractionF(1,18)=5.16p=0.04F(1,20)=10.24p=0.01
Between-Genotype Comparisons
Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Drug (between-subjects)
% FPS
GenotypeF(3,86)=1.17p=0.91
DrugF(1,86)=16.69p<0.001
Genotype x Drug InteractionF(3,86)=2.44p=0.07
2. Vogel Conflict Test
Pretest (Unpunished) Drinking
One-Way ANOVA; Factor: Genotype (between-subjects)
Number of licks
GenotypeF(3,63)=0.63p=0.60
Test (Punished) Drinking
Two-Way Factorial ANOVA; Factors: Genotype (between-subjects), Drug (between-subjects)
Number of licks
GenotypeF(3,59)=1.00p=0.40
DrugF(1,59)=14.57p<0.001
Genotype x Drug InteractionF(3,59)=1.21p=0.31
Table 4

Omnibus statistical tests of measured parameters in in vivo LFP recordings collected from ventral and dorsal hippocampus.

https://doi.org/10.7554/eLife.14120.021
VENTRAL HIPPOCAMPUS
1. Peak Theta Frequency
Two-Way ANOVA; Factors: Stimulation intensity (within-subjects), Time before/after drug (within-subjects)
α2F/Fα2CA1KO
Stimulation intensityF(4,32)=9.13p<0.001F(4,24)=18.12p<0.001
Time after drugF(2,32)=22.98p<0.001F(2,24)=37.01p<0.001
Stimulation x TimeF(8, 32)=4.27p<0.001F(8,24)=9.07p<0.001
α2CA3KOα2DGKO
Stimulation intensityF(4,32)=8.14p<0.001F(4,32)=3.84P=0.02
Time after drugF(2,32)=6.14P=0.02F(2,32)=2.47P=0.15
Stimulation x TimeF(8, 32)=3.14P=0.01F(8, 32)=6.63p<0.001
2. Stimulation Intensity – Theta Frequency Slope
One-Way ANOVA; Factor: Genotype (between-subjects)
30min post-diazepam60min post-diazepam
GenotypeF(3,15)=3.94p=0.03F(3,73)=0.84p=0.48
3. Normalized Power
One-Way ANOVA; Factor: Genotype (between-subjects)
60min post-diazepam
GenotypeF(3,15)=4.65p=0.02
DORSAL HIPPOCAMPUS
4. Peak Theta Frequency
Two-Way ANOVA; Factors: Stimulation intensity (within-subjects), Time before/after drug (within-subjects)
α2F/Fα2CA1KO
Stimulation intensityF(4,32)=8.19p<0.001F(4,32)=5.29p=0.01
Time after drugF(2,32)=31.65p<0.001F(2,32)=3.03p<0.11
Stimulation x TimeF(8,32)=8.87p=0.003F(8,32)=1.89p=0.10
α2CA3KOα2DGKO
Stimulation intensityF(4,40)=19.19p<0.001F(4,32)=8.86p<0.001
Time after drugF(2,40)=27.08p<0.001F(2,32)=9.25p=0.01
Stimulation x TimeF(8,40)=4.30p<0.001F(8,32)=2.39p=0.04
5. Stimulation Intensity – Theta Frequency Slope
One-Way ANOVA; Factor: Genotype (between-subjects)
30min post-diazepam60min post-diazepam
GenotypeF(3,17)=2.97p=0.14F(3,17)=4.32p=0.02
6. Normalized Power
One-Way ANOVA; Factor: Genotype (between-subjects)
60min post-diazepam
GenotypeF(3,15)=1.02p=0.41

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  1. Elif Engin
  2. Kiersten S. Smith
  3. Yudong Gao
  4. David Nagy
  5. Rachel A. Foster
  6. Evgeny Tsvetkov
  7. Ruth Keist
  8. Florence Crestani
  9. Jean-Marc Fritschy
  10. Vadim Y. Bolshakov
  11. Mihaly Hajos
  12. Scott A. Heldt
  13. Uwe Rudolph
(2016)
Modulation of anxiety and fear via distinct intrahippocampal circuits
eLife 5:e14120.
https://doi.org/10.7554/eLife.14120