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Cellular resolution circuit mapping in mouse brain with temporal-focused excitation of soma-targeted channelrhodopsin

  1. Christopher A Baker  Is a corresponding author
  2. Yishai M Elyada
  3. Andres Parra-Martin
  4. McLean Bolton  Is a corresponding author
  1. Max Planck Florida Institute for Neuroscience, United States
  2. Max Planck Institute for Neuroscience, United States
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Cite as: eLife 2016;5:e14193 doi: 10.7554/eLife.14193

Abstract

We describe refinements in optogenetic methods for circuit mapping that enable measurements of functional synaptic connectivity with single neuron resolution. By expanding a two-photon beam in the imaging plane using the temporal focusing method and restricting channelrhodopsin to the soma and proximal dendrites, we are able to reliably evoke action potentials in individual neurons, verify spike generation with GCaMP6s, and determine the presence or absence of synaptic connections with patch-clamp electrophysiological recording.

Article and author information

Author details

  1. Christopher A Baker

    Disorders of Neural Circuit Function, Max Planck Florida Institute for Neuroscience, Jupiter, United States
    For correspondence
    christopher.baker@mpfi.org
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon 0000-0002-0604-8449
  2. Yishai M Elyada

    Functional Architecture of the Cerebral Cortex, Max Planck Florida Institute for Neuroscience, Jupiter, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Andres Parra-Martin

    Functional Architecture of the Cerebral Cortex, Max Planck Institute for Neuroscience, Jupiter, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. McLean Bolton

    Disorders of Neural Circuit Function, Max Planck Florida Institute for Neuroscience, Jupiter, United States
    For correspondence
    mclean.bolton@mpfi.org
    Competing interests
    The authors declare that no competing interests exist.

Funding

Max Planck Florida Institute

  • Christopher A Baker
  • Yishai M Elyada
  • Andres Parra-Martin
  • McLean Bolton

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health, and all animals were handled according to protocols approved by the Institutional Animal Care and Use Committee of the Max Planck Florida Institute for Neuroscience.

Reviewing Editor

  1. Michael Häusser, University College London, United Kingdom

Publication history

  1. Received: January 5, 2016
  2. Accepted: August 14, 2016
  3. Accepted Manuscript published: August 15, 2016 (version 1)
  4. Version of Record published: August 26, 2016 (version 2)

Copyright

© 2016, Baker et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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