A transcriptomic atlas of the mouse vomeronasal organ reveals novel receptor co-expression patterns and provides a framework for understanding sensory neuron lineage and receptor specification.
The spatial autocorrelation method Local Moran’s I was used for image segmentation to delineate pixel clusters representing congruous objects with fuzzy borders, in this case potassium ion channel clusters.
A single-nucleus atlas of bone repair uncovers the trajectories of periosteal skeletal stem/progenitor cells in response to fracture and identifies injury-induced fibrogenic cells as intermediate osteochondroprogenitors and key paracrine regulators.
While monitoring neuronal activity in freely moving Drosophila is challenging, HI-FISH enables brain-wide mapping of active neurons and reveals behavior-specific neural activity.
A deep learning-based system objectively and reliably scores the Rey–Osterrieth complex figure test, enhancing the assessment of visual memory deficits from hand-drawn images in clinical settings.
John H Day, Catherine M Della Santina ... Laurie A Boyer
A simple and accessible method for high-throughput super-resolution fluorescence microscopy that is compatible with commonly used staining practices, 96-well cell culture plates, and confocal microscopes.
The automated workflow significantly enhances the precision and scalability of cell cycle analysis, enabling high-throughput studies in both non-adherent and adherent cells for advanced cancer research and drug development.
Mighten C Yip, Mercedes M Gonzalez ... Craig R Forest
Patch-walking is a novel automated patch clamp approach for finding synaptic connections in brain tissue, yielding 80–92% more probed connections than traditional approaches.
A novel pair of isobaric crosslinking reagents is described, which allow relative quantification of crosslinker-modified peptides during mass spectrometry analysis for comparative structural studies of proteins and protein complexes.
Adding suppressors of spontaneous differentiation enables precise control of human induced pluripotent stem cell (hiPSC) status in suspension culture conditions and leads to scalable and automated cell therapy using hiPSCs.
