Andrea Cuentas-Condori, Patricia Chanabá-López ... Daniel A Colón-Ramos
Endogenous tagging and conditional silencing of vesicular transporters reveal widespread, conserved co-transmission, and enable causal dissection of transmitter-specific synaptic function, linking molecular identity to circuit dynamics and behavior in vivo.
Transcriptomic profiling of the developing human outflow tract and adult aortic valves reveals that adult valve cells retain distinct embryonic molecular signatures.
Simultaneous tagging of multiple genes with different fluorophores provides a proof of concept for the scalability of tagging all genes in the Caenorhabditis elegans genome.
Giovanni Diana, B Semihcan Sermet ... David A DiGregorio
A Bayesian particle Gibbs framework enables unbiased spike time inference with millisecond resolution and jointly estimates uncertainties in both spike timing and model parameters from fast calcium imaging.
The Tapenade pipeline enables single-cell, whole-mount 3D quantification in dense multilayer organoids, linking spatial gene co-expression and nuclear deformation to emerging tissue-scale organization.
Mary L Phillips, Nicolai T Urban ... Ryohei Yasuda
Multiplexed spectral fingerprinting through a single implanted GRIN lens enables projection-resolved functional imaging of up to nine neuronal populations in freely behaving animals without post hoc histology.
Real-time nanopore transcriptomics enables early detection of expression changes and sample quality, offering a rapid, cost-effective strategy for experimental quality control and transcriptomic analysis.
Emerson M Carmona, William N Zagotta, Sharona E Gordon
A fluorescent noncanonical amino acid enables structural measurements across full-length human voltage-gated proton channel (Hv1) and reveals zinc-dependent conformational changes.
