Giovanni Diana, B Semihcan Sermet ... David A DiGregorio
A Bayesian particle Gibbs framework enables unbiased spike time inference with millisecond resolution and jointly estimates uncertainties in both spike timing and model parameters from fast calcium imaging.
The Tapenade pipeline enables single-cell, whole-mount 3D quantification in dense multilayer organoids, linking spatial gene co-expression and nuclear deformation to emerging tissue-scale organization.
Mary L Phillips, Nicolai T Urban ... Ryohei Yasuda
Multiplexed spectral fingerprinting through a single implanted GRIN lens enables projection-resolved functional imaging of up to nine neuronal populations in freely behaving animals without post hoc histology.
Real-time nanopore transcriptomics enables early detection of expression changes and sample quality, offering a rapid, cost-effective strategy for experimental quality control and transcriptomic analysis.
Emerson M Carmona, William N Zagotta, Sharona E Gordon
A fluorescent noncanonical amino acid enables structural measurements across full-length human voltage-gated proton channel (Hv1) and reveals zinc-dependent conformational changes.
Victoria Mischley, Johannes Maier ... John Karanicolas
PPIscreenML is a rigorously benchmarked method that uses AlphaFold2 to screen for interacting protein pairs, and it provides superior performance to other available methods.
New mouse genetic tools, roxCre and loxCre, facilitate the study of gene function in cells of interest, allowing for simultaneous and efficient gene knockout and precise lineage tracing in vivo.
Identification of each animal in a collective becomes possible even when individuals are never all visible simultaneously, enabling faster and more accurate analysis of collective behavior.
