Tools and Resources

A Tools and Resources article allows authors to publish the details of new experimental techniques, datasets, software tools, and other resources.

Latest articles

    1. Biochemistry
    2. Cell Biology

    Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS

    Tony Ly et al.
    A detailed analysis of protein abundance and phosphorylation changes across mitotic subphases and interphase in asynchronously growing human cells has been enabled by combining FACS with quantitative MS-based proteomics.
    1. Neuroscience

    Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)

    Lin Cong et al.
    The integration of a novel high spatiotemporal resolution volume imaging technique and a fast 3D tracking system allows capturing whole brain neural activities in a freely behaving larval zebrafish.
    1. Neuroscience

    Two-photon calcium imaging of the medial prefrontal cortex and hippocampus without cortical invasion

    Masashi Kondo et al.
    Establishment of two-photon imaging with a 1100-nm laser, which underfills the objective's back aperture, detects activity of multiple neurons in the prelimbic area and hippocampal CA1 region of the intact mouse brain.
    1. Epidemiology and Global Health

    A case study for a psychographic-behavioral segmentation approach for targeted demand generation in voluntary medical male circumcision

    Sema K Sgaier et al.
    A novel, hybrid segmentation technique reveals human heterogeneity in barriers towards a behavior, and is applied to characterize distinct segments in voluntary medical male circumcision.
    1. Genes and Chromosomes

    A synthetic biology approach to probing nucleosome symmetry

    Yuichi Ichikawa et al.
    A novel system for expression of asymmetric nucleosomes in vivo enables mechanistic studies on histone modification readouts in the cell.
    1. Developmental Biology and Stem Cells
    2. Genes and Chromosomes

    lncRNA requirements for mouse acute myeloid leukemia and normal differentiation

    M Joaquina Delás et al.
    A comprehensive analysis of long noncoding RNAs in the hematopoietic system reveals their dynamic regulation and a loss-of-function screen identifies some required for leukemia progression and involved in normal myeloid differentiation.
    1. Neuroscience

    Automated long-term recording and analysis of neural activity in behaving animals

    Ashesh K Dhawale et al.
    A new automated system for recording and analyzing neural activity in behaving animals over months-long time-scales offers new perspectives on how neural circuits underlie processes such as learning, development, and recovery from brain injury.
    1. Computational and Systems Biology
    2. Genomics and Evolutionary Biology

    MicroCT-based phenomics in the zebrafish skeleton reveals virtues of deep phenotyping in a distributed organ system

    Matthew Hur et al.
    Deep skeletal phenotyping in adult zebrafish reveals enhanced sensitivity of phenomic patterns in discriminating mutant populations.