Tools and Resources

A Tools and Resources article allows authors to publish the details of new experimental techniques, datasets, software tools, and other resources.

Latest articles

    1. Neuroscience

    Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice

    Vasiliki-Ilya Gargareta et al.
    Comparing humans and mice, the protein composition of central nervous system myelin and the transcriptional profiles of myelin-producing oligodendrocytes are largely similar with respect to abundant structural myelin constituents but show divergent expression of a range of other distinct genes.
    1. Microbiology and Infectious Disease

    A novel lineage-tracing mouse model for studying early MmuPV1 infections

    Vural Yilmaz et al.
    A novel mouse model which permits tracking of papillomavirus infected cells in mouse stratified epithelia during early infection by means of genetic reporter activation.
    1. Genetics and Genomics

    Boosting targeted genome editing using the hei-tag

    Thomas Thumberger et al.
    Increased efficiency of targeted genome editing by simple addition of a bipartite-optimized tag at the N- and C-termini of effector proteins (Cas9 and cytosine-to-thymine base editor) is applicable in organismo and cell culture.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Development and characterization of new tools for detecting poly(ADP-ribose) in vitro and in vivo

    Sridevi Challa et al.
    Poly(ADP-ribose) (PAR) Trackers (PAR-Ts) are optimized PAR sensors that allow the detection and quantification of PAR levels in extracts, living cells, and living tissues with high sensitivity, and with temporal and spatial precision, in many different experimental and biological systems.
    1. Neuroscience

    The Digital Brain Bank, an open access platform for post-mortem imaging datasets

    Benjamin C Tendler et al.
    The Digital Brain Bank (open.win.ox.ac.uk/DigitalBrainBank) provides curated post-mortem imaging datasets for neuroanatomy, neuropathology, and comparative neuroanatomy investigations, with the first release containing 21 distinctive whole-brain diffusion MRI datasets, alongside microscopy and complementary MRI modalities.
    1. Neuroscience

    Generation of a CRF1-Cre transgenic rat and the role of central amygdala CRF1 cells in nociception and anxiety-like behavior

    Marcus M Weera et al.
    A novel transgenic rat for studying the role of specific corticotropin-releasing factor type-1 receptor-expressing cell populations in physiology and behavior.
    1. Computational and Systems Biology

    Comprehensive and unbiased multiparameter high-throughput screening by compaRe finds effective and subtle drug responses in AML models

    Morteza Chalabi Hajkarim et al.
    compaRe can be used to optimize cytometric high-parameter immunophenotypic characterization of heterogeneous cell populations.
    1. Biochemistry and Chemical Biology

    Effector membrane translocation biosensors reveal G protein and βarrestin coupling profiles of 100 therapeutically relevant GPCRs

    Charlotte Avet et al.
    Development, validation, and use of an effector membrane translocation biosensor platform reveals G protein coupling selectivity signatures for 100 GPCRs that range from remarkable selectivity to full promiscuity toward the different G protein subtypes.