Tools and Resources

A Tools and Resources article allows authors to publish the details of new experimental techniques, datasets, software tools, and other resources.

Latest articles

    1. Biochemistry
    2. Cell Biology

    Affimer proteins are versatile and renewable affinity reagents

    Christian Tiede et al.
    The Affimer technology represents renewable binding reagents for molecular biology, which provides research scientists and industry with an alternative to the recently criticised use of animal-produced antibodies.
    Tools and Resources
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    1. Genes and Chromosomes
    2. Genomics and Evolutionary Biology

    Simultaneous measurement of chromatin accessibility, DNA methylation, and nucleosome phasing in single cells

    Sebastian Pott
    Establishment of a method that measures multiple aspects of chromatin organization in single cells with the possible application to study regulatory processes in heterogeneous samples.
    Tools and Resources
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genes and Chromosomes
    2. Neuroscience

    Longitudinal imaging of Caenorhabditis elegans in a microfabricated device reveals variation in behavioral decline during aging

    Matthew A Churgin et al.
    The WorMotel, a novel microfabricated device for automated longitudinal imaging of aging in large numbers of Caenorhabditis elegans, reveals that long and short lived strains vary in their behavioral decline during aging in a similar way to the long and short lived individuals in a wild type population.
    Tools and Resources Updated
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Neuroscience

    Enhanced FIB-SEM systems for large-volume 3D imaging

    C Shan Xu et al
    Enabling complete neuronal circuit reconstruction and whole-cell imaging of eukaryotes at less than 10 nm isotropic resolution.
    Tools and Resources Updated
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Dye-enhanced visualization of rat whiskers for behavioral studies

    Jacopo Rigosa et al
    1. Cell Biology
    2. Computational and Systems Biology

    Functional mapping of yeast genomes by saturated transposition

    Agnès H Michel et al
    A new method maps functional and structural features of yeast genomes with unprecedented ease and throughput, which allows identification of protein domains at the genome scale.
    Tools and Resources Updated
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology
    2. Neuroscience

    Optocontrol of glutamate receptor activity by single side-chain photoisomerization

    Viktoria Klippenstein et al
    Introduction of single photoswitchable unnatural amino acid into a neuronal receptor provides reversible, rapid and robust control of its activity by light, representing an important contribution to the fast expanding field of optopharmacology.
    Tools and Resources
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells

    Dissection of zebrafish shha function using site-specific targeting with a Cre-dependent genetic switch

    Kotaro Sugimoto et al
    A versatile invertible gene trap vector is generated and inserted into a defined genomic locus in zebrafish via homologous recombination, demonstrating an efficient genetic approach to performing conditional knockout analysis.
    Tools and Resources
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf