Katharina MC Klee, Michael W Hess ... Lukas A Huber
Applying a CRISPR knockout screen in polarized intestinal epithelial cells and subsequent phenotypic analyses identified various genes, of which several were previously not associated with epithelial homeostasis, to be relevant for proper epithelial polarization, apical cargo transport, and endo/lysosomal morphology.
Theodore J Zwang, Rachel E Bennett ... Bradley T Hyman
Conditions for multiplexed antibody labeling in mouse brain tissue translates to effective labeling in human brain tissue when samples are prepared similarly.
A collection of 22 nanobodies against 11 different Drosophila sarcomeric protein epitopes was generated, validated in stainings and for some transgenic fly lines were generated to monitor the dynamics of the sarcomeric proteins in muscles in vivo.