Tools and Resources

Latest articles

1. 1. Neuroscience

   FluoEM, virtual labeling of axons in 3-dimensional electron microscopy data for long-range connectomics

   Florian Drawitsch et al.
2. 1. Biochemistry and Chemical Biology

   Efficient analysis of mammalian polysomes in cells and tissues using Ribo Mega-SEC

   Harunori Yoshikawa et al.

   Translationally active polysomes from mammalian cells/tissues are successfully separated with rapidity, high efficiency and exceptional reproducibility by a SEC-based approach, an accessible alternative to the conventional sucrose density gradient analysis.
3. 1. Biochemistry and Chemical Biology
   2. Chromosomes and Gene Expression

   VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells

   Chitra Mohan et al.
4. 1. Genetics and Genomics

   An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila

   David Li-Kroeger et al.
5. 1. Cell Biology
   2. Computational and Systems Biology

   A quantitative approach for analyzing the spatio-temporal distribution of 3D intracellular events in fluorescence microscopy

   Thierry Pécot et al.

   QuantEv is a fully automatic and semi-parametric method that allows quantitative analysis of the spatio-temporal distribution of complex molecular trafficking objects at the scale of the whole cell.
6. 1. Cancer Biology
   2. Computational and Systems Biology

   Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes

   Jia-Ren Lin et al.

   t-CyCIF can be used to collect spatially-encoded, multiparametric data from fixed and embedded research or clinical specimens making it possible to probe the organization of tumors and tissues at a single-cell level.
7. 1. Immunology and Inflammation

   Capturing change in clonal composition amongst single mouse germinal centers

   Daniel J Firl et al.

   Repeated interrogation of single germinal centers in vivo reveals a more fluid landscape than expected and unlocks new lines of investigation.
8. 1. Chromosomes and Gene Expression
   2. Computational and Systems Biology

   Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells

   Raghuvir Viswanatha et al.

   Pooled CRISPR knockout screening in Drosophila cells enables high-resolution, genome-wide functional genomic comparisons in cell-lines across vast evolutionary distance.
9. 1. Biochemistry and Chemical Biology
   2. Structural Biology and Molecular Biophysics

   Visualizing conformational dynamics of proteins in solution and at the cell membrane

   Sharona E Gordon et al.

   ACCuRET is a flexible new method for measuring the structural dynamics of proteins in solution and membrane proteins in their native environment.
10. 1. Microbiology and Infectious Disease

    Parvovirus Minute Virus of Mice Interacts with Sites of Cellular DNA Damage to Establish and Amplify its Lytic Infection

    Kinjal Majumder et al.