A robot capable of automatically obtaining blind whole cell patch clamp recordings from multiple neurons simultaneously guides four interacting electrodes in a coordinated fashion, avoiding mechanical coupling in the brain.
The ability to rapidly stain for any combination of genes in intact tissue with automated quantification of transcripts in individual cells and spatial re-mapping affords new insights into lung biology, and will greatly accelerate progress in scientific and medical research.
Dissection of a cis-regulatory element (CRM) in its native chromosomal context using CRISPR/Cas9 editing and novel 'Active Genetics' reveals new features of CRM function and insights into how such regulatory elements change during evolution.
A statistical approach for predicting non-active site residues responsible for allostery, cooperativity, or other subtle but functionally important interactions is described and applied to various protein families.
Engineered E3 ubiquitin ligases are utilized to elucidate mechanisms underlying ubiquitin regulation of membrane proteins, and to achieve robust post-translational functional knockdown of ion channels.