Tools and Resources

Latest articles

1. 1. Biochemistry
   2. Cell Biology

   Affimer proteins are versatile and renewable affinity reagents

   Christian Tiede et al.

   The Affimer technology represents renewable binding reagents for molecular biology, which provides research scientists and industry with an alternative to the recently criticised use of animal-produced antibodies.
2. 1. Genes and Chromosomes
   2. Genomics and Evolutionary Biology

   Simultaneous measurement of chromatin accessibility, DNA methylation, and nucleosome phasing in single cells

   Sebastian Pott

   Establishment of a method that measures multiple aspects of chromatin organization in single cells with the possible application to study regulatory processes in heterogeneous samples.
3. 1. Genes and Chromosomes
   2. Neuroscience

   Longitudinal imaging of Caenorhabditis elegans in a microfabricated device reveals variation in behavioral decline during aging

   Matthew A Churgin et al.

   The WorMotel, a novel microfabricated device for automated longitudinal imaging of aging in large numbers of Caenorhabditis elegans, reveals that long and short lived strains vary in their behavioral decline during aging in a similar way to the long and short lived individuals in a wild type population.
4. 1. Cell Biology
   2. Neuroscience

   Enhanced FIB-SEM systems for large-volume 3D imaging

   C Shan Xu et al

   Enabling complete neuronal circuit reconstruction and whole-cell imaging of eukaryotes at less than 10 nm isotropic resolution.
5. 1. Developmental Biology and Stem Cells
   2. Plant Biology

   Live tracking of moving samples in confocal microscopy for vertically grown roots

   Daniel von Wangenheim et al
6. 1. Neuroscience

   Dye-enhanced visualization of rat whiskers for behavioral studies

   Jacopo Rigosa et al
7. 1. Cell Biology
   2. Computational and Systems Biology

   Functional mapping of yeast genomes by saturated transposition

   Agnès H Michel et al

   A new method maps functional and structural features of yeast genomes with unprecedented ease and throughput, which allows identification of protein domains at the genome scale.
8. 1. Developmental Biology and Stem Cells
   2. Neuroscience

   A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells

   Sonal Nagarkar-Jaiswal et al
9. 1. Biophysics and Structural Biology
   2. Neuroscience

   Optocontrol of glutamate receptor activity by single side-chain photoisomerization

   Viktoria Klippenstein et al

   Introduction of single photoswitchable unnatural amino acid into a neuronal receptor provides reversible, rapid and robust control of its activity by light, representing an important contribution to the fast expanding field of optopharmacology.
10. 1. Developmental Biology and Stem Cells

    Dissection of zebrafish shha function using site-specific targeting with a Cre-dependent genetic switch

    Kotaro Sugimoto et al

    A versatile invertible gene trap vector is generated and inserted into a defined genomic locus in zebrafish via homologous recombination, demonstrating an efficient genetic approach to performing conditional knockout analysis.