By integrating multiplexed error-robust fluorescence in situ hybridization (MERFISH), deep learning, optical sectioning, and protocol optimization, thick-tissue 3D MERFISH allows volumetric single-cell transcriptome imaging of thick-tissue samples, broadening the scope of applications of spatial genomics.
Paolo Petazzi, Telma Ventura ... Antonella Fidanza
A novel tool is used to assess gene function in vitro by targeted endogenous transcriptional activation to unveil novel players in development and disease.
The software package Ais simplifies automated segmentation in cryo-electron tomography, enabling users to segment, detect particles, and visualize data in a streamlined and accessible workflow.
Bruno Bockorny, Lakshmi Muthuswamy ... Senthil K Muthuswamy
Proteomic profiling of extracellular vesicle proteins from patients with benign and malignant pancreatic diseases offers a valuable resource for identifying biomarkers for pancreatic cancer detection.
The spatial autocorrelation method Local Moran’s I was used for image segmentation to delineate pixel clusters representing congruous objects with fuzzy borders, in this case potassium ion channel clusters.
A transcriptomic atlas of the mouse vomeronasal organ reveals novel receptor co-expression patterns and provides a framework for understanding sensory neuron lineage and receptor specification.
A single-nucleus atlas of bone repair uncovers the trajectories of periosteal skeletal stem/progenitor cells in response to fracture and identifies injury-induced fibrogenic cells as intermediate osteochondroprogenitors and key paracrine regulators.
While monitoring neuronal activity in freely moving Drosophila is challenging, HI-FISH enables brain-wide mapping of active neurons and reveals behavior-specific neural activity.
A deep learning-based system objectively and reliably scores the Rey–Osterrieth complex figure test, enhancing the assessment of visual memory deficits from hand-drawn images in clinical settings.
John H Day, Catherine M Della Santina ... Laurie A Boyer
A simple and accessible method for high-throughput super-resolution fluorescence microscopy that is compatible with commonly used staining practices, 96-well cell culture plates, and confocal microscopes.
