Tools and Resources

Latest articles

1. 1. Structural Biology and Molecular Biophysics

   Screening of candidate substrates and coupling ions of transporters by thermostability shift assays

   Homa Majd et al.
2. 1. Chromosomes and Gene Expression
   2. Neuroscience

   Species and cell-type properties of classically defined human and rodent neurons and glia

   Xiao Xu et al.

   Gene expression and epigenetic profiling of defined cell types in the central nervous system of mouse, rat, and human reveals inter-species and inter-individual differences.
3. 1. Computational and Systems Biology
   2. Genetics and Genomics

   New insights into the cellular temporal response to proteostatic stress

   Justin Rendleman et al.

   A time-resolved analysis of protein and RNA concentrations and interactions during proteostasis stress highlights the dominant role of translation regulation and a shift of energy metabolism.
4. 1. Genetics and Genomics

   Generation of a versatile BiFC ORFeome library for analyzing protein–protein interactions in live Drosophila

   Johannes Bischof et al.

   Generation of a new fly line library for analysing protein–protein interactions in vivo.
5. 1. Developmental Biology
   2. Genetics and Genomics

   Epigenetic age-predictor for mice based on three CpG sites

   Yang Han et al.

   Site-specific DNA methylation analysis at three CG dinucleotides by pyrosequencing provides a reliable measure for epigenetic aging of mice.
6. 1. Neuroscience

   FluoEM, virtual labeling of axons in three-dimensional electron microscopy data for long-range connectomics

   Florian Drawitsch et al.

   Methodology to annotate the multiple origins of axonal projections in dense electron microscopy data of mammalian nervous tissue without the need of chemical label conversion is reported.
7. 1. Neuroscience

   In vivo detection of optically-evoked opioid peptide release

   Ream Al-Hasani et al.

   In vivo detection of optically evoked endogenous opioid peptides reveals anatomically distinct release profiles.
8. 1. Chromosomes and Gene Expression
   2. Neuroscience

   Transcriptional and epigenomic landscapes of CNS and non-CNS vascular endothelial cells

   Mark F Sabbagh et al.

   Genome-wide integration of transcriptome, accessible chromatin, and DNA methylome data from vascular endothelial cells lays the foundation for understanding the gene regulatory circuits that generate organ-specific vascular specialization.
9. 1. Developmental Biology
   2. Genetics and Genomics

   Efficient single-copy HDR by 5’ modified long dsDNA donors

   Jose Arturo Gutierrez-Triana et al.

   The 5' modification of the donor template facilitates highly efficient Crispr targeted homologous recombination and at the same time favors single copy integration.
10. 1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells

    Chitra Mohan et al.

    An in vivo disulfide crosslinking assay shows preferential disassembly of nucleosomes with two H2A.Z histones by transcription machinery in yeast and conjugation to one or two ubiquitin moieties in human cells.