Tools and Resources

A Tools and Resources article allows authors to publish the details of new experimental techniques, datasets, software tools, and other resources.

Latest articles

    1. Neuroscience

    Kilohertz transcranial magnetic perturbation (kTMP) as a new non-invasive method to modulate cortical excitability

    Ludovica Labruna, Christina Merrick ... Daniel Sheltraw
    kTMP is a novel, non-invasive brain stimulation method that, via continuous magnetic induction, safely and effectively increases cortical excitability without any tactile sensation.
    1. Neuroscience

    Automatic and accurate reconstruction of long-range axonal projections of single-neuron in mouse brain

    Lin Cai, Taiyu Fan ... Shaoqun Zeng
    A novel point assignment method achieves 80% f1-score in single-neuron reconstruction across broad brain regions, enabling high-throughput brain-wide projection mapping.
    1. Genetics and Genomics

    Identifying in vivo genetic dependencies of melanocyte and melanoma development

    Sarah Perlee, Yilun Ma ... Richard M White
    A zebrafish Cas9 knock-in model enables lineage-specific gene disruption to uncover genetic dependencies in development and disease.
    1. Computational and Systems Biology

    Unsupervised pipeline for the identification of cortical excitatory and inhibitory neurons in high-density multielectrode arrays with ground-truth validation

    Eloise Giraud, Michael Lynn ... Jean-Philippe Thivierge
    A novel software tool and ground-truth optogenetic validation is provided to automatically detect putative excitatory and inhibitory cells in neuronal recordings performed with large-scale multielectrode arrays.
    1. Cell Biology
    2. Neuroscience

    The promise and peril of comparing fluorescence lifetime in biology revealed by simulations

    Pingchuan Ma, Peter Chen ... Yao Chen
    A versatile computational framework realistically simulates fluorescence lifetime data in biological systems, enhancing rigor by quantifying signal variance and identifying limitations, and enabling innovations such as multiplexed dynamic biosensor imaging.
    1. Developmental Biology

    Long-term live imaging, cell identification and cell tracking in regenerating crustacean legs

    Çağrı Çevrim, Béryl Laplace-Builhé ... Michalis Averof
    Live imaging allows tracking cellular behaviours and cell fates during the entire course of leg regeneration, spanning approximately one week, in a crustacean model.
    1. Biochemistry and Chemical Biology

    Phage-displayed synthetic library and screening platform for nanobody discovery

    Baolong Xia, Ah-Ram Kim ... Norbert Perrimon
    A phage-displayed synthetic library and screening platform offer a streamlined, cost-effective solution for nanobody discovery.
    1. Genetics and Genomics

    Specific modulation of CRISPR transcriptional activators through RNA-sensing guide RNAs in mammalian cells and zebrafish embryos

    Oana Pelea, Sarah Mayes ... Tatjana Sauka-Spengler
    RNA-sensing guide RNAs provide a programmable platform for conditional, sequence-specific activation of CRISPR-Cas9 upon RNA detection in diverse biological contexts.
    1. Neuroscience

    A computational pipeline to track chromatophores and analyze their dynamics

    Johann Ukrow, Mathieu DM Renard ... Gilles Laurent
    Computer code is provided to track the size and shape of deformable pigment cells in the skin of cephalopods from high-resolution video images.
    1. Computational and Systems Biology
    2. Neuroscience

    A SMARTTR workflow for multi-ensemble atlas mapping and brain-wide network analysis

    Michelle Jin, Simon O Ogundare ... Christine Ann Denny
    A new scalable workflow for analyzing multiple ensemble datasets incorporates SMARTTR, an R package supporting network analysis, visualization, and the import of external datasets.