Tools and Resources

A Tools and Resources article allows authors to publish the details of new experimental techniques, datasets, software tools, and other resources.

Latest articles

    1. Computational and Systems Biology

    Applying causal discovery to single-cell analyses using CausalCell

    Yujian Wen, Jielong Huang ... Hao Zhu
    Based on benchmarking various methods and analyzing multiple real scRNA-seq datasets, a computational platform/workflow and a set of tips for best practices are developed for analyzing causal interactions or relationships in single cells.
    1. Neuroscience

    retro-Tango enables versatile retrograde circuit tracing in Drosophila

    Altar Sorkaç, Rareș A Moșneanu ... Gilad Barnea
    retro-Tango, developed and validated in multiple circuits in Drosophila melanogaster, is established as a genetically encoded, transsynaptic labeling system in the retrograde direction.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Gene editing and scalable functional genomic screening in Leishmania species using the CRISPR/Cas9 cytosine base editor toolbox LeishBASEedit

    Markus Engstler, Tom Beneke
    LeishBASEedit enables gene editing in Leishmania without requiring DNA double-strand breaks, homologous recombination, or donor DNA, thereby facilitating loss-of-function screens via delivery of plasmid libraries and regardless of limitations due to gene copy number variations and/or lack of RNAi components.
    1. Cell Biology

    pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells

    Payam E Farahani, Xiaoyu Yang ... Jared E Toettcher
    pYtags are novel biosensors that can be used to measure the activity of a receptor tyrosine kinase of interest in live cells with high spatiotemporal resolution and are applied to reveal rapid activity dynamics of EGFR/ErbB2 signaling.
    1. Genetics and Genomics

    Rapid and precise genome engineering in a naturally short-lived vertebrate

    Claire N Bedbrook, Ravi D Nath ... Anne Brunet
    Rapid and efficient CRISPR/Cas9-mediated knock-in of fluorescent reporters at various genomic loci enables cell- and tissue-specific expression and establishes the short-lived African killifish as a vertebrate system for precise genetic engineering at scale.
    1. Immunology and Inflammation
    2. Neuroscience

    MHC class I and MHC class II reporter mice enable analysis of immune oligodendroglia in mouse models of multiple sclerosis

    Em P Harrington, Riley B Catenacci ... Peter A Calabresi
    Oligodendroglia express major histocompatibility complex (MHC) pathways in response to inflammation and MHC reporter mice allow for the investigation of MHC molecule expressing cells in vivo.
    1. Computational and Systems Biology
    2. Medicine

    Local generation and efficient evaluation of numerous drug combinations in a single sample

    Vlad Elgart, Joseph Loscalzo
    Engineered local heterogeneity in drug concentration is used as a tool to encode drug treatment regimens and to predict the macroscopic cellular response to drug perturbations.
    1. Neuroscience

    Enhanced functional detection of synaptic calcium-permeable AMPA receptors using intracellular NASPM

    Ian Coombs, Cécile Bats ... Mark Farrant
    Intracellular NASPM, unlike the widely used spermine, fully blocks outward currents through calcium-permeable AMPA-type glutamate receptors, enabling an improved functional readout for this physiologically important receptor subtype in neurons.