Tools and Resources

A Tools and Resources article allows authors to publish the details of new experimental techniques, datasets, software tools, and other resources.

Latest articles

    1. Developmental Biology and Stem Cells
    2. Genomics and Evolutionary Biology

    Mapping the mouse Allelome reveals tissue-specific regulation of allelic expression

    Daniel Andergassen et al.
    Allele-specific expression due to genetic differences, X-chromosome inactivation or genomic imprinting, varies dynamically throughout development, and may be explained by allele-specific differences in stability or the actions of tissue-specific enhancers.
    1. Cell Biology

    The human cytoplasmic dynein interactome reveals novel activators of motility

    William B Redwine et al.
    Using a proximity-dependent labeling approach in living cells, the human cytoplasmic dynein-1 interactome was identified and a new family of dynein activators, ninein and ninein-like, was discovered.
    1. Plant Biology

    ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology

    Valentina De Col et al.
    The monitoring of ATP dynamics in living plants using fluorescent protein biosensor provides evidence for the complex relationship between cellular energy status and growth.
    1. Neuroscience

    Dye-enhanced visualization of rat whiskers for behavioral studies

    Jacopo Rigosa et al.
    A simple fluorescent dye method allows visualization of whiskers, facilitating studies of rodent tactile behavior.
    1. Neuroscience

    SynEM, automated synapse detection for connectomics

    Benedikt Staffler et al.
    1. Developmental Biology and Stem Cells
    2. Plant Biology

    Live tracking of moving samples in confocal microscopy for vertically grown roots

    Daniel von Wangenheim et al.
    A set of tools to image and track growing plant roots in optimized growing conditions within a vertical stage confocal microscope will open up new possibilities in research of root development.
    1. Developmental Biology and Stem Cells
    2. Neuroscience

    A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells

    Sonal Nagarkar-Jaiswal et al.
    A conditional gene inactivation system that permits the tracking of protein expression, and creates fluorescently-labelled mutant cells in both developing and adult Drosophila tissues.
    1. Biochemistry
    2. Cell Biology

    Affimer proteins are versatile and renewable affinity reagents

    Christian Tiede et al.
    The Affimer technology represents renewable binding reagents for molecular biology, which provides research scientists and industry with an alternative to the recently criticised use of animal-produced antibodies.