Based on benchmarking various methods and analyzing multiple real scRNA-seq datasets, a computational platform/workflow and a set of tips for best practices are developed for analyzing causal interactions or relationships in single cells.
retro-Tango, developed and validated in multiple circuits in Drosophila melanogaster, is established as a genetically encoded, transsynaptic labeling system in the retrograde direction.
LeishBASEedit enables gene editing in Leishmania without requiring DNA double-strand breaks, homologous recombination, or donor DNA, thereby facilitating loss-of-function screens via delivery of plasmid libraries and regardless of limitations due to gene copy number variations and/or lack of RNAi components.
Payam E Farahani, Xiaoyu Yang ... Jared E Toettcher
pYtags are novel biosensors that can be used to measure the activity of a receptor tyrosine kinase of interest in live cells with high spatiotemporal resolution and are applied to reveal rapid activity dynamics of EGFR/ErbB2 signaling.
Rapid and efficient CRISPR/Cas9-mediated knock-in of fluorescent reporters at various genomic loci enables cell- and tissue-specific expression and establishes the short-lived African killifish as a vertebrate system for precise genetic engineering at scale.
Em P Harrington, Riley B Catenacci ... Peter A Calabresi
Oligodendroglia express major histocompatibility complex (MHC) pathways in response to inflammation and MHC reporter mice allow for the investigation of MHC molecule expressing cells in vivo.
Engineered local heterogeneity in drug concentration is used as a tool to encode drug treatment regimens and to predict the macroscopic cellular response to drug perturbations.
Intracellular NASPM, unlike the widely used spermine, fully blocks outward currents through calcium-permeable AMPA-type glutamate receptors, enabling an improved functional readout for this physiologically important receptor subtype in neurons.
