Multi-body refinement in RELION allows high-resolution reconstruction and visualisation of molecular motions in cryo-EM single-particle data sets with continuous forms of structural heterogeneity.
A developmental transcriptomics resource from Drosophila flight muscles quantifies the transcriptional dynamics during muscle morphogenesis and identifies three ordered phases of sarcomere morphogenesis.
Fiducial-less tomography on single particle cryoEM samples reveals that most particles are adsorbed to the air-water interface and allows for researchers to diagnose and solve sample, grid, ice thickness, collection, and processing issues.
Binding of a charged small molecule to a membrane protein changes the amplitude of the apparent membrane capacitance by changing the charge density at the surfaces of the membrane.
A family of fluorescent biosensors for nicotinamide adenine dinucleotides allows quantification of these cofactors in live cells with spatio-temporal resolution.
A broadly applicable method that faithfully preserves genetically labeled cellular structures for 3D electron microscopy (EM) and correlated light and electron microscopy (CLEM).
