Physical determinants of vesicle mobility and supply at a central synapse
Abstract
Encoding continuous sensory variables requires sustained synaptic signalling. At several sensory synapses, rapid vesicle supply is achieved via highly mobile vesicles and specialized ribbon structures, but how this is achieved at central synapses without ribbons is unclear. Here we examine vesicle mobility at excitatory cerebellar mossy fibre synapses which sustain transmission over a broad frequency bandwidth. Fluorescent recovery after photobleaching in slices from VGLUT1Venus knock-in mice reveal 75% of VGLUT1-containing vesicles have a high mobility, comparable to that at ribbon synapses. Experimentally constrained models establish hydrodynamic interactions and vesicle collisions are major determinants of vesicle mobility in crowded presynaptic terminals. Moreover, models incorporating 3D reconstructions of vesicle clouds near active zones (AZs) predict the measured releasable pool size and replenishment rate from the reserve pool. They also show that while vesicle reloading at AZs is not diffusion-limited at the onset of release, diffusion limits vesicle reloading during sustained high-frequency signalling.
Article and author information
Author details
Funding
Wellcome Trust (95667)
- Robin Angus Silver
European Research Council (294667)
- Robin Angus Silver
European Research Council (293681)
- Zoltan Nusser
Magyar Tudományos Akadémia (Momentum Grant, Lendület, LP2012-29)
- Zoltan Nusser
European Commission (LSHM-CT-2005-019055)
- Zoltan Nusser
- Robin Angus Silver
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Christian Rosenmund, Charité-Universitätsmedizin Berlin, Germany
Ethics
Animal experimentation: Animal experiments were conducted in strict accordance with the United Kingdom Home Office Animals Scientific Procedures Act of 1986, and approved by the UCL ethics review board. All mice were anaesthetized with ketamine or isoflurane during surgical procedures.
Version history
- Received: February 9, 2016
- Accepted: August 14, 2016
- Accepted Manuscript published: August 19, 2016 (version 1)
- Accepted Manuscript updated: August 23, 2016 (version 2)
- Version of Record published: September 15, 2016 (version 3)
Copyright
© 2016, Rothman et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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