(A,D) Representative time course of SNr neuron firing rate with application of NSCC blocker flufenamic acid (FFA; 100 μM) (A) and 2-aminoethoxydiphenylborane (2-APB; 100 μM) (D). Inset in panel a shows 1 s recordings before and after FFA. Scale bar: 100 pA, 200 ms. (B,E) Scatter plot of mean firing rate of SNr neurons before and after FFA application (30.7 ± 3.7 versus 15.2 ± 2.0 spikes/s; paired t-test; p = 0.00013; n = 9) (B) and before and after 2-APB (23.6 ± 2.8 versus 3.9 ± 1.9 spikes/s; paired t-test; p<0.0001; n = 8) (E). Box plots indicate the population median, interquartile range, and maximum and minimum values. (C,F) Dose response of FFA (C) and 2-APB (F) on firing rate. Data are fit with a Hill function (red line). (G) Mean basal firing rate of SNr neurons from wild-type mice (WT; 37.3 ± 2.7; n = 20), mice lacking TRPC3 (TRPC3 KO; 28.6 ± 4.3; n = 10) and mice lacking all seven TRPC channels (hepta-TRPC KO; 28.7 ± 3.4; n = 7). Error bars indicate s.e.m. n.s.p>0.05; one-way ANOVA. (H) Representative time course of SNr firing rate with application of TRPC channel blocker SKF-96365 (100 μM). (I) Mean firing rate of SNr neurons before and after SKF-96365 application (30.0 ± 3.1 versus 30.7 ± 3.2 spikes/s; paired t-test; p = 0.19; n = 9), and box plot summary of the population statistics before and after SKF-96365. For panels B, E, I: n.s.p>0.05; ***p<0.001.