1. Developmental Biology
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The Glide/Gcm fate determinant controls initiation of collective cell migration by regulating Frazzled

  1. Tripti Gupta
  2. Arun Kumar
  3. Cattenoz Pierre
  4. K VijayRaghavan
  5. Angela Giangrande  Is a corresponding author
  1. Institut de Génétique et de Biologie Moléculaire et Cellulaire, France
  2. University of California, Riverside, United States
  3. Tata Institute for Fundamental Research, India
Research Article
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Cite this article as: eLife 2016;5:e15983 doi: 10.7554/eLife.15983

Abstract

Collective migration is a complex process that contributes to build precise tissue and organ architecture. Several molecules involved in cell interaction control collective migration, but what their precise role is and how is their expression finely tuned to orchestrate the different steps of the process is poorly understood. Here we show that the timely and threshold expression of the Netrin receptor Frazzled triggers the initiation of glia migration in the Drosophila wing. Frazzled expression is induced by the Glide/Gcm transcription factor in a dose dependent manner. Thus, the glial determinant also regulates the efficiency of collective migration. NetrinB but not NetrinA serves as a chemoattractant and Unc5 contributes as a repellant Netrin receptor for glia migration. Our model includes strict spatial localization of a ligand, a cell autonomously acting receptor and a fate determinant that act coordinately to direct glia towards their final destination.

Article and author information

Author details

  1. Tripti Gupta

    Department of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
    Competing interests
    No competing interests declared.
  2. Arun Kumar

    Department of Entomology, University of California, Riverside, Riverside, United States
    Competing interests
    No competing interests declared.
  3. Cattenoz Pierre

    Department of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
    Competing interests
    No competing interests declared.
  4. K VijayRaghavan

    Department of Developmental Biology and Genetics, Tata Institute for Fundamental Research, Bangalore, India
    Competing interests
    K VijayRaghavan, Senior editor, eLife.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-4705-5629
  5. Angela Giangrande

    Department of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
    For correspondence
    angela@igbmc.fr
    Competing interests
    No competing interests declared.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-6278-5120

Funding

CEFIPRA-4403-1 (graduate student fellowship)

  • K VijayRaghavan
  • Angela Giangrande

Agence Nationale de la Recherche (international award)

  • Angela Giangrande

Fondation pour la Recherche Médicale (labelisation)

  • Angela Giangrande

ARC Centre of Excellence for Coherent X-Ray Science (Projet grant)

  • Angela Giangrande

Ligue Contre le Cancer (Grant regional)

  • Angela Giangrande

USIAS

  • Angela Giangrande

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Hugo J Bellen, Baylor College of Medicine, United States

Publication history

  1. Received: March 11, 2016
  2. Accepted: October 12, 2016
  3. Accepted Manuscript published: October 14, 2016 (version 1)
  4. Version of Record published: November 8, 2016 (version 2)

Copyright

© 2016, Gupta et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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