(A) The knockdown of Nkx2-5, Gata4, and Tbx5 in eCMs was analysed by Western blotting. siNkx2-5, Nkx2-5 siRNA; siTbx5, Tbx5 siRNA; siGata4, Gata4 siRNA; WT, wild type. (B, C) Enriched gene ontologies in eCMs (for up-regulated genes: siNkx2-5,89 genes; siTbx5, 58 genes; siGata4, 182 genes. for down-regulated genes: siNkx2-5, 12 genes; siTbx5, 9 genes; siGata4, 13 genes) and E9.5 Nkx2-5-/- hearts (up-regulated genes, 200 genes; down-regulated genes, 90 genes) are shown. N.D., not detected. The p value is plotted on the x-axis.