Klf5 regulates muscle differentiation by directly targeting muscle-specific genes in cooperation with MyoD in mice

Abstract
Data availability
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Abstract

Krüppel-like factor 5 (Klf5) is a zinc-finger transcription factor that controls various biological processes, including cell proliferation and differentiation. We show that Klf5 is also an essential mediator of skeletal muscle regeneration and myogenic differentiation. During muscle regeneration after injury (cardiotoxin injection), Klf5 was induced in the nuclei of differentiating myoblasts and newly formed myofibers expressing myogenin in vivo. Satellite cell-specific Klf5 deletion severely impaired muscle regeneration, and myotube formation was suppressed in Klf5-deleted cultured C2C12 myoblasts and satellite cells. Klf5 knockdown suppressed induction of muscle differentiation-related genes, including myogenin. Klf5 ChIP-seq revealed that Klf5 binding overlaps that of MyoD and Mef2, and Klf5 physically associates with both MyoD and Mef2. In addition, MyoD recruitment was greatly reduced in the absence of Klf5. These results indicate that Klf5 is an essential regulator of skeletal muscle differentiation, acting in concert with myogenic transcription factors such as MyoD and Mef2.

Data availability

The following data sets were generated

1. Yumiko Oishi
(2016) Klf5 regulates muscle differentiation via directly targeted muscle-specific genes in cooperation with MyoD
Publicly available at the NCBI Gene Expression Omnibus (accession no: GSE80812).

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE80812

The following previously published data sets were used

1. Cao Y
2. Yao Z
(2010) Genome-wide analysis of gene expression during differentiation in C2C12 cells
Publicly available at the NCBI Gene Expression Omnibus (accession no: GSE20059).

http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE20059
1. Sebastian S
2. Dilworth FJ
(2013) Alternate exon switching establishes a tissue-specific transcription factor to mediate temporal activation of gene expression during differentiation
Publicly available at the NCBI Gene Expression Omnibus (accession no: GSE43223).

http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE43223

Article and author information

Author details

Shinichiro Hayashi

Department of Cellular and Molecular Medicine, Tokyo Medical and Dental University, Tokyo, Japan

Competing interests
The authors declare that no competing interests exist.
Ichiro Manabe

Department of Aging Research, Graduate School of Medicine, Chiba University, Chiba, Japan

Competing interests
The authors declare that no competing interests exist.
Yumi Suzuki

Department of Cellular and Molecular Medicine, Tokyo Medical and Dental University, Tokyo, Japan

Competing interests
The authors declare that no competing interests exist.
Frédéric Relaix

INSERM U955 IMRB-E10 UPEC, ENVA, EFS, 8 rue du general Sarrail, 94010 Creteil, Creteil, France

Competing interests
The authors declare that no competing interests exist.
Yumiko Oishi

Department of Cellular and Molecular Medicine, Tokyo Medical and Dental University, Tokyo, Japan

For correspondence
yuooishi-circ@umin.ac.jp

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-4761-0952

Funding

Japan Society for the Promotion of Science (26882020)

Shinichiro Hayashi

Japan Society for the Promotion of Science (25H10)

Yumiko Oishi

Nakatomi Foundation

Shinichiro Hayashi

Uehara Memorial Foundation

Shinichiro Hayashi

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: Animal experimentation was approved by the Experimental Animal Care and Use Committee of Tokyo Medical and Dental University　(approval numbers 2013-027C12 and 0170280C).

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.