(A–D) Quantification of autophagosomes (AP; GFP punctae) in the intestine (A), body-wall muscle (B), pharynx (C), and nerve-ring neurons (D) at Days 1, 3, 5, 7, or 10 of adulthood in wild type (WT; dotted black same as Figure 1F–I), daf-2(e1370) (green), and glp-1(e2141) (blue). Data are the mean ± SEM of ≥20 animals combined from three independent experiments per time point. ***/###p < 0.0001, **/##p < 0.001, and */#p < 0.01. *** for WT control vs glp-1/daf-2 on each day; ### for WT/glp-1/daf-2 control at Days 1, 3, 5, 7, or 10 vs. WT/glp-1/daf-2 control at Day 1 by Poisson regression. (E–H’) Quantification of APs in WT (black line) and daf-2(e1370) animals (E–H, green) and WT and glp-1(e2141) animals (E’–H’, blue) injected with DMSO (control, black/dark green/dark blue) or Bafilomycin A (BafA, gray/light green/light blue) at Day 1 or Day 7 of adulthood. Tissues examined were the intestine (E,E’), body-wall muscle (F,F’), pharynx (G,G’), and nerve-ring neurons (H,H’). Data for WT animals are the same in (E–H) and (E’–H’). Data are the mean ± SEM of ≥25 animals combined from at least three experiments. Day 7 was omitted for neurons due to a counting issue of this time point. , WT control vs. daf-2 or glp-1 control at Days 1 and 7; *, daf-2 or glp-1 control vs. daf-2 or glp-1 + BafA at Days 1 and 7, #, daf-2 or glp-1 control at Day 7 vs. daf-2 or glp-1 control at Day 1. ***//###p < 0.0001, **//##p < 0.001, *//#p < 0.01 by Poisson regression.