Selecting the most appropriate time points to profile in high-throughput studies
- Carnegie Mellon University, United States
- University of Alabama at Birmingham, United States
- University of California, San Diego, United States
- Yale University, United States
Abstract
Biological systems are increasingly being studied by high throughput profiling of molecular data over time. Determining the set of time points to sample in studies that profile several different types of molecular data is still challenging. Here we present the Time Point Selection (TPS) method that solves this combinatorial problem in a principled and practical way. TPS utilizes expression data from a small set of genes sampled at a high rate. As we show by applying TPS to study mouse lung development, the points selected by TPS can be used to reconstruct an accurate representation for the expression values of the non selected points. Further, even though the selection is only based on gene expression, these points are also appropriate for representing a much larger set of protein, miRNA and DNA methylation changes over time. TPS can thus serve as a key design strategy for high throughput time series experiments.
Data availability
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miRNA Data of Mouse Lung DevelopementPublicly available at the Systems Biology Group, School of Computer Science, Carnegie Mellon University website'.
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Author details
Funding
National Institutes of Health (U01 HL122626)
- Ziv Bar-Joseph
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols (APN 10042) of the University of Alabama at Birmingham. All lungs were isolated immediately following euthanasia using approved protocols.
Copyright
© 2017, Kleyman et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Selecting the most appropriate time points to profile in high-throughput studies
eLife 6:e18541.
https://doi.org/10.7554/eLife.18541
