(A–C) Clec11a expression analysis by microarray, RNA-seq and qRT-PCR. Whole bone marrow cells, VE-Cadherin+ bone marrow endothelial cells, bone marrow stromal cells (Scf-GFP+CD45-Ter119-CD31- for microarray, PDGFRα+CD45-Ter119-CD31- for RNA-seq, and LepR+CD45-Ter119-CD31- for qPCR), Col2.3-GFP+CD45-Ter119-CD31- osteoblasts and hematopoietic cell populations were sorted from enzymatically dissociated femur bone marrow of two month-old mice, followed by microarray (A), RNA-seq (B) and qPCR (C) analysis. The statistical significance of differences was assessed using one-way ANOVAs with Dunnett’s multiple comparison tests (n = 2–3 mice/genotype from at least two independent experiments). (D) The position of images from femur sections. (E–H) Confocal analysis of isotype control or anti-Clec11a antibody staining in the femur metaphysis (E and F) and diaphysis (G and H) of Prrx1-Cre; tdTomato mice. Bone was imaged by second harmonic generation (SHG). Bone marrow stromal cells (Fi), osteoblasts (Fii), hypertrophic chondrocytes (Fiii) and osteocytes (H) are marked by arrows (n = 3 mice per genotype, total, from three independent experiments). (I and J) Confocal analysis of isotype control (I) or anti-Clec11a antibody staining (J) in the femur metaphysis of Lepr-Cre; tdTomato mice. LepR+ bone marrow stromal cells are marked by arrows (n = 3 mice per genotype, total, from three independent experiments). (K) Representative images of two month-old control and Clec11a-/- mice. (L) Body mass of 2 and 10 month-old mice (n = 3–8 mice per genotype, total, from at least three independent experiments for all data in panels L–T). (M) Cellularity of the bone marrow and spleen. (N–T) Flow cytometric analysis of the frequencies of myeloid cells (N), erythroid progenitors (O), T cells (P), B cells (Q), hematopoietic stem cells (R), multipotent progenitors (S) and restricted progenitors (T) in the bone marrow and spleen of Clec11a-/- mice and sex-matched littermate controls. The statistical significance of differences among genotypes was assessed using two-tailed Student’s t tests. Data represent mean ± SD: *p<0.05, **p<0.01, ***p<0.001. The source data are in Figure 1—source data 1.