A comprehensive excitatory input map of the striatum reveals novel functional organization

Abstract
Data availability
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Abstract

The striatum integrates excitatory inputs from the cortex and the thalamus to control diverse functions. Although the striatum is thought to consist of sensorimotor, associative and limbic domains, their precise demarcations and whether additional functional subdivisions exist remain unclear. How striatal inputs are differentially segregated into each domain is also poorly understood. This study presents a comprehensive map of the excitatory inputs to the mouse striatum. The input patterns reveal boundaries between the known striatal domains. The most posterior striatum likely represents a novel functional subdivision, and the dorsomedial striatum integrates highly heterogeneous, multimodal inputs. The complete thalamo-cortico-striatal circuit loop is also presented, which reveals that the thalamic subregions innervated by the basal ganglia preferentially interconnect with motor-related cortical areas. Optogenetic experiments show the subregion-specific heterogeneity in the synaptic properties of striatal inputs from both the cortex and the thalamus. This projectome will guide functional studies investigating diverse striatal functions.

Data availability

The following previously published data sets were used

1. Oh et al.
(2014) AIBS Mouse Brain Connectivity Atlas
publicaly available for download.

http://connectivity.brain-map.org
(2014) Thalamus-whole brain projection dataset
Downsized images are freely available for download. Full size images are available from T. Mao lab upon request.

http://digitalcollections.ohsu.edu/projectionmap

Article and author information

Author details

Barbara J Hunnicutt

Vollum Institute, Oregon Health and Science University, Portland, United States

Competing interests
The authors declare that no competing interests exist.
Bart C Jongbloets

Vollum Institute, Oregon Health and Science University, Portland, United States

Competing interests
The authors declare that no competing interests exist.
William T Birdsong

Vollum Institute, Oregon Health and Science University, Portland, United States

Competing interests
The authors declare that no competing interests exist.
Katrina J Gertz

Vollum Institute, Oregon Health and Science University, Portland, United States

Competing interests
The authors declare that no competing interests exist.
Haining Zhong

Vollum Institute, Oregon Health and Science University, Portland, United States

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-7109-4724
Tianyi Mao

Vollum Institute, Oregon Health and Science University, Portland, United States

For correspondence
mao@ohsu.edu

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-3532-8319

Funding

National Institute of Neurological Disorders and Stroke (R01NS081071)

Tianyi Mao

National Institute of Mental Health (DP2OD008425)

Haining Zhong

National Science Foundation

Barbara J Hunnicutt

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: All animal experiments were conducted according to National Institutes of Health guidelines for animal research and were approved by the Institutional Animal Care and Use Committee (IACUC protocol number: IS00003542).

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.