(A) A cartoon picture and (B) GFP images show the morphology of RME neurons. Cell bodies of four RME neurons, labeled as D (RME dorsal), V (RME ventral), L (RME left) and R (RME right), form a …
(A) Quantification of the fractions of SNB-1::GFP signals in RME D/V neurites (green), axonal boutons (blue) and cell bodies (red) in control and unc-1(lf) animals. The total intensity of SNB-1::GFP …
(A) Images showed that loss-of-function in unc-1(e580) (without unc-30(ju32) background) caused the misaccumulation of SNB-1::GFP puncta in both RME nerve ring processes and RME D neurites, Scar …
(A) Quantification of SNB-1::GFP puncta distribution defects in unc-1(lf) mutants and rescue transgenic strains. Promoters used to drive expression of unc-1 cDNA are: Pdpy-30 in all tissues; Punc-33 …
(A) A confocal image shows the relative positions of GLR cells (Pink) and RME neurons (Green). GLR cells were labeled by expression of Pgly-18::mCherry, and RME neurons were labeled by expression of …
(A) Time kymographs generated from a 40-s movie of EBP-2::GFP dynamics in control, unc-1(e580) and unc-7 unc-9 animals. (B) Quantification of percentage of orientation in RME D/V neurites in control …
Confocal images (A) and quantification (B) of EMTB::GFP signals in RME D/V neurites. n = 15. Data are shown as mean ± SD. Student test, NS: no significant difference. Pink-colored neurons are AIY …
(A) Loss-of-function of cdk-5 induces microtubule polarity defects in RME D/V neurites to a similar degree as those in unc-1(lf) animals. cdk-5: n = 81. Values that differ significantly from wild …
(A) Gel images and (B) quantification results show that calcium antagonist BAPTA-AM and Calpain inhibitor PD150606 suppress the cleavage of CDKA-1/p35. Three independent experiments were performed, …
(A) Quantification of SNB-1::GFP distribution defects in unc-1(ju1057), slo-1 and unc-1(ju1057);slo-1. (B) Quantification of SNB-1::GFP distribution defects in animals expressing calbindin D28K …
At the early developmental stage, calcium influxes from GLR cells to RME neurons through gap junctions to induce activation of the CDK-5 pathway, which guides microtubule assembly and axon …
Supplemental Table 1, Strain list.
Supplemental Table 2, Plasmid list.