(A) Diagram of the experimental protocol. The GFP+ NP-specific B cells were transferred into non-responding C57BL recipients, which were subsequently immunized by footpad with NP-CGG in CFA. …
GFP+ NP-specific B cells were transferred into WT C57BL recipients, followed by footpad immunization of NP-CGG in CFA. Shown are representative immunofluorescence histology of LN sections (n = 3 …
RelB+ cells identified and summarized in Figure 1 (B) (C) and (D) show intracellular staining of RelB, which co-localizes mostly with nuclear BCL6 and IRF4. (A) Representaive intracellular RelB …
GFP+ NP-specific B cells were transferred into WT C57BL recipients, followed by footpad immunization of NP-CGG in CFA. Mice recieved either 30 μg hamster Ig isotype control or blocking anti-CD40L …
(A–E) Flow cytometric analysis of draining LN Ag-specific B cells. GFP+ NP-specific B cells were transferred into C57BL/6 recipients, which were subsequently immunized by footpad with NP-CGG in CFA. …
GFP+ NP-specific B cells were transferred into C57BL/6 recipients, followed by f.p. immunization of NP-CGG in CFA. Popliteal draining LNs were stained for flow cytometric analysis. (A–B) …
(A) Diagram of the experimental protocol. CFSE-labeled NP-specific B cells were transferred into CD45.1+ congenic recipients, followed by f.p. immunization with NP-CGG in CFA. Popliteal draining LNs …
Shown are representative plots of BCL6 expression relative to CFSE dilution. BCL6lo IRF4+ cells (light blue dots), GC precursors (red dots) and GC B cells (blue dots) are overlaid onto total …
(A–B) Flow cytometric analysis for cell cycle and apoptosis. NP-specific B cells were transferred into C57BL/6 recipients, followed by f.p. immunization of NP-CGG in CFA. 3.5 days p.i, popliteal …
(A) Diagram of the experimental protocol. CFSE-labeled NP-specific B cells were transferred into CD45.1+ congenic recipients, followed by f.p. immunization of NP-CGG of various doses (either 1.5 μg …
(A) Diagram of the experimental protocol. 3 days after the initial immunization, recipient mice received an additional injection of either soluble NP-CGG, agonistic anti-CD40 (clone: FGK), PBS or …
(A–F) Diagram of the experimental protocol (A). By day 3 post-immunization, 20 μg αCD4 or αCD40L was further injected locally to delete CD4 T cells or block CD40 ligation on B cells. Popliteal …
(A) Diagram of the experimental protocol. (B) Percent GC B cells among transferred B cells 2 days after depletionof CD4 T cells or blockade of CD40L. Two independent experiments were carried with n =…
(A) Schematic of the culture system using primary murine B cells. (B) Overlay of BCL6 expression in live B cells under different culture conditions as indicated. (C) Contour plots of BCL6 expression …
After fixation and intracellular staining with antibody, cultured cells were further hybridized with probes for murine bcl6 to allow for the detection of both BCL6 protein and bcl6 RNA at the single …
(A) Bcl6yfp/+ B cells were used for in vitro cell cultures. YFP-Bcl6 hi and lo cells in live B cells from day 3 culture with a continuous exposure of aCD40+IL-4/21 were gated based on control …