Mapping cortical mesoscopic networks of single spiking cortical or sub-cortical neurons
- University of British Columbia, Canada
- Capital Medical University, China
Abstract
Understanding the basis of brain function requires knowledge of cortical operations over wide-spatial scales, but also within the context of single neurons. In vivo, wide-field GCaMP imaging and sub-cortical/cortical cellular electrophysiology were used in mice to investigate relationships between spontaneous single neuron spiking and mesoscopic cortical activity. We make use of a rich set of cortical activity motifs that are present in spontaneous activity in anesthetized and awake animals. A mesoscale spike-triggered averaging procedure allowed the identification of motifs that are preferentially linked to individual spiking neurons by employing genetically targeted indicators of neuronal activity. Thalamic neurons predicted and reported specific cycles of wide-scale cortical inhibition/excitation. In contrast, spike-triggered maps derived from single cortical neurons yielded spatio-temporal maps expected for regional cortical consensus function. This approach can define network relationships between any point source of neuronal spiking and mesoscale cortical maps.
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Author details
Funding
Canadian Institutes of Health Research (MOP-12675)
- Timothy H Murphy
Canadian Institutes of Health Research (FDN-143209)
- Timothy H Murphy
International Alliance of Translational Neuroscience (N/A)
- Dongsheng Xiao
Brain Canada, Canadian Neurophotonics Platform
- Jeff M LeDue
- Timothy H Murphy
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: Animal protocols (A13-0336 and A14-0266) were approved by the University of British Columbia Animal Care Committee and conformed to the Canadian Council on Animal Care and Use guidelines.
Copyright
© 2017, Xiao et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Mapping cortical mesoscopic networks of single spiking cortical or sub-cortical neurons
eLife 6:e19976.
https://doi.org/10.7554/eLife.19976
