Mexicans, on average, had a greater proportion of Native American ancestry than Puerto Ricans; Puerto Ricans had a greater proportion of European and African ancestry. Mixed and other Latinos were …
(A) Distribution of the first 10 principal coordinates of the methylation data. Plots in the diagonal show the univariate distribution; those in the lower left triangle show bivariate relationship …
(A) Association between ethnicity and principal coordinate 7. (B) Association between Native American ancestry proportion and PC7, colored by ethnicity. Native American ancestry explains …
(B) Violin plot showing one such locus, cg19145607. Mexicans are relatively hypermethylated compared to Puerto Ricans (p=1.4×10–19). (C) Plot showing the association between Native American ancestry …
(A) Venn diagram showing the effect of adjustment for ancestry on the association between ethnicity and methylation. The components of the diagram represent the number of CpG’s that remained …
(A) Manhattan plot showing the associations between genomic ancestry and methylation at individual CpG loci. (B) Plot showing one such locus, cg04922029, and genomic African ancestry, showing a …
(A) Association between cg04922029 on the DARC locus and African ancestry, color coded by ethnic group. There is near perfect correlation between the two. (B) Association between SNPs located within …
Baseline characteristics of GALA II participants with methylation data, stratified by ethnicity. Continuous variables are reported with inter-quartile range in brackets.
Mexican | Puerto rican | Mixed latino | Other latino | |
---|---|---|---|---|
n | 276 | 220 | 16 | 61 |
Males (%) | 125 (45.3%) | 127 (57.7%) | 6 (37.5%) | 28 (45.9%) |
Age | 11.4 [9.3: 14.7] | 12.3 [10.4: 14.2] | 11.8 [10.7: 14.9] | 11.8 [10: 15.7] |
Asthma cases (%) | 124 (44.9%) | 147 (66.8%) | 9 (56.3%) | 31 (50.8%) |
Ancestry (n = 524) | ||||
African | 4.3% [2.9%: 6.0%) | 22.8% [16.6%: 29.4%) | 8.5% [5.6%: 19.2%) | 12.3% [6.3%: 25.8%) |
Native American | 55.4% [44.5%: 65.7%) | 11.2% [9.8%: 13%) | 31.5% [20.9%: 45.6%) | 32.8% [10.4%: 49.3%) |
European | 40.5% [29.9%: 50.2%) | 65.7% [59.2%: 71%) | 50.5% [44.6%: 57.6%) | 48.9% [40%: 58.5%) |
Recruitment Site | ||||
Chicago | 140 (50.7%) | 15 (6.8%) | 11 (68.9%) | 15 (24.6%) |
New York | 18 (6.5%) | 10 (4.5%) | 1 (6.3%) | 23 (37.7%) |
Puerto Rico | 0 | 193 (87.7%) | 0 | 0 |
San Francisco | 78 (28.3%) | 0 | 2 (12.5%) | 23 (37.7%) |
Houston | 40 (14.5%) | 2 (0.9%) | 2 (12.5%) | 5 (8.2%) |
Cell Counts (estimated) | ||||
Granulo cytes | 51.2% [46.0%: 55.7%) | 51.6% [46.8%: 57%) | 51% [43.6%: 57.2%) | 49.1% [43.8%: 55.8%) |
Lympho cytes | 41.9% [36.9%: 46.6%) | 41.8% [36.9%: 46.5%) | 41.9% [36.1%: 51.6%) | 43.9% [36.8%: 49.6%) |
Mono cytes | 7.1% [5.8%: 8.3%) | 6.74% [5.74%: 8.24%) | 6.6% [5.7%: 7.6%) | 7.4% [6.2%: 8.6%) |
Proportion of variance in methylation explained by ethnicity and ancestry. Numbers represent the median and interquartile range.
Component | All CpG’s associated with ethnicity (n = 916) | CpG’s associated with ethnicity after adjusting for ancestry (n = 314) | CpG’s whose association with ethnicity is explained by ancestry (n = 520) |
---|---|---|---|
Joint | 6.8% (4.5% to 10%) | 6.2% (4.4% to 8.8%) | 7.8% (5.3% to 11.1%) |
Ethnicity | 1.7% (0.78% to 3.0%) | 3.5% (2.2% to 5.1%) | <1% |
Ancestry | 4.2% (1.8% to 8.3%) | 1.8% (0.8% to 4.0%) | 6.6% (4.0% to 10.2%) |
(A) Methylation principal components and ethnicity and ancestry. (B) Significant associations between ethnicity and methylation. (C) Effect of adding cubic spline ancestry terms to the association between ethnicity and methylation. (D) Effect of adding quadratic and cubic ancestry terms to the association between ethnicity and methylation. (E) Effect of adding genetic principal components 3–10 to the association between ethnicity and methylation. (F) Significant associations between ethnicity and methylation (p<1.6×10–7), and effect of adjustment for ancestry on the association of ethnicity and methylation, excluding participants of ‘Mixed Latino’ ethnicity. (G) Association of ethnicity and methylation in loci previously associated with maternal smoking during pregnancy. (H) Significant associations between ethnicity and methylation loci previously associated with environmental exposures. (I) Significant associations between global ancestry and methylation.