Novel adverse outcome pathways revealed by chemical genetics in a developing marine fish
- Institute of Marine Research, Norway
- Northwest Fisheries Science Center, United States
- University of Oslo, Norway
Abstract
Crude oil spills are a worldwide ocean conservation threat. Fish are particularly vulnerable to the oiling of spawning habitats, and crude oil causes severe abnormalities in embryos and larvae. However, the underlying mechanisms for these developmental defects are not well understood. Here we explore the transcriptional basis for four discrete crude oil injury phenotypes in the early life stages of the commercially important Atlantic haddock (Melanogrammus aeglefinus). These include defects in 1) cardiac form and function, 2) craniofacial development, 3) ionoregulation and fluid balance, and 4) cholesterol synthesis and homeostasis. Our findings suggest a key role for intracellular calcium cycling and excitation-transcription coupling in the dysregulation of heart and jaw morphogenesis. Moreover, the disruption of ionoregulatory pathways sheds new light on buoyancy control in marine fish embryos. Overall, our chemical-genetic approach identifies initiating events for distinct adverse outcome pathways and novel roles for individual genes in fundamental developmental processes.
Data availability
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Data from: Supporting datasets for: Novel adverse outcome pathways revealed by chemical genetics in a developing marine fishAvailable at Dryad Digital Repository under a CC0 Public Domain Dedication.
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Sequence dataPublicly available at the NCBI Sequence Read Archive (accession no: PRJNA287744).
Article and author information
Author details
Funding
Research Council of Norway (Project no. 234367)
- Elin Sorhus
- John Patrick Incardona
- Tomasz Furmanek
- Nathaniel L Scholz
- Sonnich Meier
- Rolf Brudvik Edvardsen
VISTA foundation (Project no. 6161)
- Elin Sorhus
Institute of Marine Research (Project no. 14236)
- Elin Sorhus
- Tomasz Furmanek
- Sonnich Meier
- Rolf Brudvik Edvardsen
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Marianne Bronner, California Institute of Technology, United States
Ethics
Animal experimentation: All animal experiments within the study were approved by NARA, the governmental Norwegian Animal Research Authority (http://www.fdu.no/fdu/, reference number 2012/275334-2). All methods were performed in accordance with approved guidelines.
Version history
- Received: August 26, 2016
- Accepted: January 20, 2017
- Accepted Manuscript published: January 24, 2017 (version 1)
- Version of Record published: February 10, 2017 (version 2)
Copyright
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
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Novel adverse outcome pathways revealed by chemical genetics in a developing marine fish
eLife 6:e20707.
https://doi.org/10.7554/eLife.20707
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