(A) Schematic of the optogenetic approach. DRN neurons are infected with AAV2/9-Dio-ChR2-EYFP. In SERT-Cre mice, 5-HT neurons will express ChR2-YFP (green cells) and can be photoactivated with blue …
(A) Position tracking of example WT (top) and SERT-Cre (bottom) mice. All positions visited in the session are shown in gray; the positions visited during each 3 s stimulation periods are shown for …
(A) Schematic of the accelerating rotarod assay. (B) Latency to fall for one example SERT-Cre mouse, with randomly interspersed stimulated trials. (C) Average latency to fall, showing learning and …
(A) Schematic drawing of the LocoMouse apparatus. Water deprived animals walk freely across a glass corridor connected to two boxes with water ports. A mirror below at 45° angle allows a single …
(A) Polar plots indicating the phase of the step cycle in which each limb enters stance, aligned to stance onset of front-right paw (FR, red), for the population of SERT-Cre mice (mean, N = 7). …
(A) Position tracks of an example SERT-Cre mouse, depicting the main areas of the open field: corners (dark pink), periphery (light blue), center (light pink) and edges (gray). Filled circles …
(A) Position tracks for an example SERT-Cre mouse for sessions before (pre), during (T1, T2, T3) and after (post) photostimulation. Red square indicates the ROI in which photostimulation occurred …
(A) Schematic diagram of the photostimulation protocol, as in Figure 1D; a total of 270 s of 20 Hz stimulation is delivered over a 30 min session. (B) Experimental protocol. Group 1 (G1, 3 SERT-Cre …
(A) Difference in speed during the pre-stimulation interval between sessions n+1 and n-1 is plotted against the stimulation effect on day n (delta speed, difference between post- and pre-stimulation …
Also shown are the behavioral state (red), photostimulation condition (on/off, filled/empty blue square respectively), and speed (green rectangle, normalized to the maximum value in that session).