Wild-type (A) and klp10ARNAi (B) GSCs stained for Asl-YFP (green, centrosome), Fas III (red, hub), Vasa (blue). GSCs are indicated by dotted circles. Arrows indicate proximal (mother) centrosomes; arrowheads indicate distal (daughter) centrosomes. Hub (*). Bar: 5 µm. (C) Quantification of centrosome size in control (N = 13) and klp10ARNAi (N = 30 GSC-L, N = 15 GSC-S) GSCs, control (N = 14) and klp10ARNAi (N = 15) SGs, and control (N = 9) and klp10ARNAi (N = 11) spermatocytes (SC). (D) PACT-GFP (NGT40>PACT-GFP) labels the elongated, proximal centrosome. γ-tubulin and Fas III (red), Vasa (blue), GFP-PACT (green). Hub (*). GSC is indicated by broken line, and the centrosomes are indicated by arrowheads. (E, F) Examples of klp10ARNAi GSC clones at 0 days (E) and seven days (F) after clone induction (hs-FLP, nos>stop>gal4, UAS-GFP, UAS-klp10ARNAi). GFP (green, klp10ARNAi GSC clones), Fas III, γ-tubulin (red). Bar: 10 µm. (G) Quantification of centrosome length in klp10ARNAi GSC clones on indicated days after clone induction. Day0 (N = 13), Day3 (N = 16), Day5 (N = 10), Day7 (N = 9) and Day8 (N = 12) were scored. Statistical analysis and graphing were performed using GraphPad prism six software. The p value (two-tailed Student’s t-test) is provided for comparison between indicated columns in C. Bars in G indicate means. Note that GSC-L and –S indicate longer and shorter centrosomes, respectively, from each GSC in C and G. Scoring of GSC centrosome size in these panels come only from GSCs whose centrosomes shows clear size difference (overall average size including GSCs without visibly elongated centrosomes is given in the text).