Coincident postsynaptic activity gates presynaptic dopamine release to induce plasticity in Drosophila mushroom bodies

  1. Kohei Ueno  Is a corresponding author
  2. Ema Suzuki
  3. Shintaro Naganos
  4. Kyoko Ofusa
  5. Junjiro Horiuchi
  6. Minoru Saitoe  Is a corresponding author
  1. Tokyo Metropolitan Institute of Medical Science, Japan
7 figures

Figures

Figure 1 with 1 supplement
Synaptic vesicle exocytosis upon stimulation of the ALs and AFV.

(A) Schematic diagram of the stimulation/recording system. (B) Fluorescent images of a representative MB-LexA,LexAop-R-GECO1/UAS-spH, GH146-GAL4 brain. (C) AL stimulation induced Ca2+ responses in …

https://doi.org/10.7554/eLife.21076.002
Figure 1—figure supplement 1
Activity of glutamatergic neurons is required for normal STM.

(A) When GMR51C07-GAL4/UAS-shits flies are trained and tested at the permissive temperature, 15 min memory is unaffected compared to controls. (B) when these flies are trained at a restrictive …

https://doi.org/10.7554/eLife.21076.003
Figure 2 with 1 supplement
NR activation can substitute for AFV stimulation during LTE induction.

(A) AFV-evoked Ca2+ responses in the MBs are significantly diminished in Nmdar1EP331 mutants. Upper panel, typical images of G-CaMP fluorescence immediately after AFV stimulation in MB-LexA, …

https://doi.org/10.7554/eLife.21076.004
Figure 2—figure supplement 1
NRs in the MBs are required for normal learning.

Knocking down NRs in the MBs by expressing different inverted repeats of Nmdar2 (Nmdar2-IR) from the OK107-GAL4 driver reduces 3 min memory compared to driver alone controls.

https://doi.org/10.7554/eLife.21076.005
D1R activation is necessary and sufficient to induce LTE.

(A) DopRf02676 mutations suppress LTE induced by AL + NMDA stimulation. Left panel, MB-LexA, LexAop-G-CaMP2/+, one-way ANOVA (F2,18 = 5.932, p<0.001). Right panel, MB-LexA, LexAop-G-CaMP2/+; DopRf026…

https://doi.org/10.7554/eLife.21076.006
DA is required for induction but not maintenance or expression of LTE.

(A) DA-induced LTE was abolished by applying 100 μM SCH23390 10 min prior to treatment with 200 μM DA in c309-GAL4; UAS-G-CaMP brains. One-way ANOVA indicates significant differences between time …

https://doi.org/10.7554/eLife.21076.007
Coincident AL stimulation and NMDA application induces DA release.

(A) Time course of spH fluorescence changes and quantification of spH responses at TH-DA terminals on the vertical lobes of the MBs upon indicated treatments. Brains were first exposed to 100 µM …

https://doi.org/10.7554/eLife.21076.008
LTE and DA release require Rut-Ac activity in the MBs.

(A) LTE induced by simultaneous AL + AFV stimulation is suppressed in rut mutants. N = 7 for both control (MB-LexA, LexAop-G-CaMP2) and rut mutants (rut2080; MB-LexA, LexAop-G-CaMP2). (B) DA release …

https://doi.org/10.7554/eLife.21076.009
DA release is restricted to coincidentally postsynaptic MB neurons.

(A) Schematic diagram of the connections between ALs, MBs, and TH-DA neurons. PNs (yellow) connect ALs only to ipsilateral MB calyces, whereas DA neurons (green) innervate MBs bilaterally. (B) …

https://doi.org/10.7554/eLife.21076.010

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