(A) Schematic diagram of the stimulation/recording system. (B) Fluorescent images of a representative MB-LexA,LexAop-R-GECO1/UAS-spH, GH146-GAL4 brain. (C) AL stimulation induced Ca2+ responses in …
(A) When GMR51C07-GAL4/UAS-shits flies are trained and tested at the permissive temperature, 15 min memory is unaffected compared to controls. (B) when these flies are trained at a restrictive …
(A) AFV-evoked Ca2+ responses in the MBs are significantly diminished in Nmdar1EP331 mutants. Upper panel, typical images of G-CaMP fluorescence immediately after AFV stimulation in MB-LexA, …
Knocking down NRs in the MBs by expressing different inverted repeats of Nmdar2 (Nmdar2-IR) from the OK107-GAL4 driver reduces 3 min memory compared to driver alone controls.
(A) DopRf02676 mutations suppress LTE induced by AL + NMDA stimulation. Left panel, MB-LexA, LexAop-G-CaMP2/+, one-way ANOVA (F2,18 = 5.932, p<0.001). Right panel, MB-LexA, LexAop-G-CaMP2/+; DopRf026…
(A) DA-induced LTE was abolished by applying 100 μM SCH23390 10 min prior to treatment with 200 μM DA in c309-GAL4; UAS-G-CaMP brains. One-way ANOVA indicates significant differences between time …
(A) Time course of spH fluorescence changes and quantification of spH responses at TH-DA terminals on the vertical lobes of the MBs upon indicated treatments. Brains were first exposed to 100 µM …
(A) LTE induced by simultaneous AL + AFV stimulation is suppressed in rut mutants. N = 7 for both control (MB-LexA, LexAop-G-CaMP2) and rut mutants (rut2080; MB-LexA, LexAop-G-CaMP2). (B) DA release …
(A) Schematic diagram of the connections between ALs, MBs, and TH-DA neurons. PNs (yellow) connect ALs only to ipsilateral MB calyces, whereas DA neurons (green) innervate MBs bilaterally. (B) …