In vivo vizualisation of Mono-ADP-ribosylation by dPARP16 upon amino-acid starvation

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Abstract

PARP catalysed ADP-ribosylation is a post-translational modification involved in several physiological and pathological processes, including cellular stress. In order to visualise both Poly-, and Mono-, ADP-ribosylation in vivo, we engineered specific fluorescent probes. Using them, we show that amino-acid starvation triggers an unprecedented display of mono-ADP-ribosylation that governs the formation of Sec body, a recently identified stress assembly that forms in Drosophila cells. We show that dPARP16 catalytic activity is necessary and sufficient for both amino-acid starvation induced mono-ADP-ribosylation and subsequent Sec body formation and cell survival. Importantly, dPARP16 catalyses the modification of Sec16, a key Sec body component, and we show that it is a critical event for the formation of this stress assembly. Taken together our findings establish a novel example for the role of mono-ADP-ribosylation in the formation of stress assemblies, and link this modification to a metabolic stress.

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Angelica Aguilera Gomez

Hubrecht Institute, Utrecht, Netherlands

Competing interests
The authors declare that no competing interests exist.
Marinke M van Oorschot

Hubrecht Institute, Utrecht, Netherlands

Competing interests
The authors declare that no competing interests exist.
Tineke Veenendaal

Department of Cell Biology, University Medical Center Utrecht, Utrecht, Netherlands

Competing interests
The authors declare that no competing interests exist.
Catherine Rabouille

Hubrecht Institute-KNAW, Utrecht, Netherlands

For correspondence
c.rabouille@hubrecht.eu

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0002-3663-9717

Funding

Netherlands Wetenschappelijke Organisatie (822-020-016)

Catherine Rabouille

Hubrecht Institute

Catherine Rabouille

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

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This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.