Binocular matching of thalamocortical and intracortical circuits in the mouse visual cortex
- Northwestern University, United States
Figures
Figure 1
Binocular matching of subthreshold membrane potential responses in layer 4 neurons.
(A) A schematic of the experimental setup. (B) Histology of a recorded neuron. Red is the neuron stained with Streptavidin-Alex Fluor 547; green is ChR2 conjugated with YFP. Left, low magnification …
Figure 2 with 2 supplements
Optogenetic isolation of thalamic inputs to layer 4 neurons in binocular V1.
(A) A schematic of optogenetic photostimulation and recording in V1. (B) Summary plot of average spike rate of deep-layer neurons before, during and after LED activation. The spike rate was …
Figure 2—figure supplement 1
Control experiments to validate optogenetic silencing of visual cortex.
(A–C) Extracellular recording showing complete optogenetic silencing of the visual cortex. Shown are a deep-layer neuron’s peri-stimulus timing histogram (PSTH) of spiking response to grating at the …
Figure 2—figure supplement 2
Scale factors for thalamo-cortical transformation in adult and young mice.
(A–B) Scale factor for thalamo-cortical transformation from contralateral (A) and ipsilateral (B) inputs in adult mice. (C) Correlation of contralateral and ipsilateral scale factor in adult mice. (D…
Figure 3 with 2 supplements
Binocular matching of thalamic input precedes that of intracortical input.
(A–B) Binocular matching of total (A1 and A2, red) and thalamic (B1 and B2, blue) EPSCs in adult mice. (A1) and (B1) show correlations of the preferred orientation between the two eyes. (A2) and (B2)…
Figure 3—figure supplement 1
Orientation difference between thalamic and total EPSCs in young mice.
(A–B) Correlation of orientation preference between thalamic and total EPSCs in young mice. (A) for contralateral and (B) for ipsilateral eye response. (C) Mean orientation difference between …
Figure 3—figure supplement 2
Relative amplitude of thalamic and total EPSCs between the two eyes over development.
(A) Mean EPSC amplitude for total and thalamic input through contralateral (black) or ipsilateral eye (grey) in adult mice. (B) Same plot as (A), in young mice between P15 and P21. (C) …
Figure 4 with 1 supplement
Development of eye-specific thalamocortical transformation.
(A–B) Correlation of orientation preference between thalamic and cortical EPSCs in adult mice. (A) for contralateral and (B) for ipsilateral pathway. (C) Scatter plots of thalamic-cortical …
Figure 4—figure supplement 1
Binocular matching of intracortical EPSCs in layer 4 neurons.
(A–B) Example of cortical EPSC traces and tuning curves. (A) Example traces of cycle-averaged thalamic (blue) and total (red) EPSCs and their point-by-point subtraction (cortical). (B) This cell’s …
