(a) A heatmap of 16,929 enhancer-like regions were selected by: accessible chromatin (ATAC-seq), coincident with H3K4me2 and H3K27ac deposition (ChIP-seq) in gene-distal positions (≥3 kb from promoters). Rows are enhancer loci, repeated for each data type in columns. Bidirectional transcription of enhancer RNAs (eRNAs) is also evident (GRO-seq). (b) The top four enriched motifs that occur in enhancers from a are shown. The transcription factor (TF) family, de novo motif matrix, percentage motif occurrence at enhancer loci versus random loci, and enrichment -logPvalues are indicated. Enrichment was calculated from 200 bp sequence, centered on chromatin accessibility. (c) The positional enrichment of the enriched motifs are shown relative to the center of the enhancer-like elements from a, where 0 bp is the center of the accessibility signal. (d) Gene expression measured by RNA-seq and limited to TFs is ranked by FPKM values to nominate the most highly expressed TFs in the AP1, ETS, SOX, and GATA families. (e, f) RNA expression, histone modifications, and super enhancer (SE) definitions are shown for: (e) ERG and (f) JUN loci. SE regions are highlighted in yellow and were defined using H3K27ac. More related data in Figure 1—figure supplements 1–3.