A high-resolution map of transcriptional repression
- MRC London Institute of Medical Sciences, United Kingdom
- Wellcome Trust - Medical Research Council Stem Cell Institute, United Kingdom
Abstract
Turning genes on and off is essential for development and homeostasis, yet little is known about the sequence and causal role of chromatin state changes during the repression of active genes. This is surprising, as defective gene silencing underlies developmental abnormalities and disease. Here we delineate the sequence and functional contribution of transcriptional repression mechanisms at high temporal resolution. Inducible entry of the NuRD-interacting transcriptional regulator Ikaros into mouse pre-B cell nuclei triggered immediate binding to target gene promoters. Rapid RNAP2 eviction, transcriptional shutdown, nucleosome invasion, and reduced transcriptional activator binding required chromatin remodeling by NuRD-associated Mi2beta/CHD4, but were independent of HDAC activity. Histone deacetylation occurred after transcriptional repression. Nevertheless, HDAC activity contributed to stable gene silencing. Hence, high resolution mapping of transcriptional repression reveals complex and interdependent mechanisms that underpin rapid transitions between transcriptional states, and elucidates the temporal order, functional role and mechanistic separation of NuRD-associated enzymatic activities.
Data availability
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A high-resolution map of transcriptional repression by IkarosPublicly available at the NCBI Gene Expression Omnibus (accession no: GSE89716).
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Brg1 activates enhancer repertoires to establish B cell identity and modulate cell growthPublicly available at the NCBI Gene Expression Omnibus (accession no: GSE66978).
Article and author information
Author details
Matthias Merkenschlager
Funding
Medical Research Council (MC-A652-5PY20)
- David Rueda
- Amanda G Fisher
- Matthias Merkenschlager
Wellcome (099276/Z/12/Z)
- Matthias Merkenschlager
UK-China Scholarships for Excellence
- Ziwei Liang
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Liang et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A high-resolution map of transcriptional repression
eLife 6:e22767.
https://doi.org/10.7554/eLife.22767
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