Dopamine signaling tunes spatial pattern selectivity in C. elegans
- Fred Hutchinson Cancer Research Center, United States
Figures
Figure 1 with 2 supplements
C. elegans exhibits spatial pattern selectivity.
(A) A schematic drawing of the polydimethylsiloxane (PDMS) chamber used in the spatial pattern preference assay. Each chamber is divided into four sections (I–IV). The PDMS chamber (designated …
Figure 1—figure supplement 1
Spatial pattern selectivity is modulated by feeding state.
(A) An illustration showing the dimensions of the quad-chamber. (B) The time course of spatial pattern selection. Spatial pattern preference index values and % of worms in pattern IV were measured …
Figure 1—figure supplement 2
Spatial pattern selectivity is an individual behavior that relies on mechanosensation and spatial context.
(A) Spatial pattern selectivity is not a social behavior. ‘Single’ – A single N2 worm was loaded into the quad-chamber, and its location was recorded at 60 min. Twenty chambers with single worms …
Figure 2
Boundary experience impacts C. elegans spatial pattern selectivity.
Crossing the boundary from pattern IV to pattern III stimulates worms to return to pattern IV. (A) The red line indicates the boundary between patterns IV and III, and the blue line serves as a …
Figure 3 with 1 supplement
Spatial pattern selectivity of C. elegans requires dopamine signaling.
(A) The requirement for dopamine biosynthesis. Mutant cat-2 worms (e1112) that lack CAT-2 tyrosine hydroxylase show impaired preference for pattern IV, which is rescued by the addition of dopamine …
Figure 3—figure supplement 1
The design strategy for a trp-4 allele (pek220).
A disruption cassette that contains gfp::tbb-2utr and Prps-0::hygR::unc-54utr was inserted into trp-4 exon 2, which results in a deletion and an early stop codon that put trp-4 out-of-frame after …
Figure 4 with 1 supplement
Hawaiian strain does not select pattern IV.
(A) The Hawaiian CB4856 strain (HW) does not show significant preference for pattern IV of the quad-chamber. The representative image was taken at 60 min after the worms were loaded into the …
Figure 4—figure supplement 1
HW worms show identical locomotory behavior in quad- and uni- chambers.
Traveling speed (A) and % pause (B) were quantified as described in Figure 1. n = 5; n.s.: not significant.
Figure 5 with 1 supplement
Polymorphisms in the mechanotransduction TRP-4 channel contribute to the variation of spatial pattern selectivity.
(A) A cartoon illustrating the amino acid substitutions (M36I, G41S, and E67G) in HW TRP-4. (B) Chromosomes I and X were linked to altered spatial pattern selectivity between N2 and HW strains. …
Figure 5—figure supplement 1
Both N2 and HW trp-4 cDNAs rescue defects in the swim-to-crawl transition of trp-4(ok1605) mutant worms.
(A) The swim-to-crawl transition was analyzed as described in previous studies (Vidal-Gadea et al., 2011). Blue: dorsal body bend. Red: ventral body bend. (B) Transgenic trp-4(ok1605) worms carrying …
Figure 6
Polymorphisms in TRP-4 alter mechanosensitivity of dopaminergic neurons.
(A) Representative traces of mechanoreceptor currents in wild type N2 and HW dopaminergic CEP neurons in response to small mechanical displacements (1 μm, 2 μm and 4 μm). Whole-cell patch clamp …
Figure 7
Natural variations in either TRP-4 or DOP-3 tune spatial pattern selectivity in C. elegans.
(A) Spatial preference of C. elegans natural isolates was quantified using regular quad-chambers. n = 12 for each strain, ***p<0.001 and **p<0.01 when compared to N2; Dunnett's multiple comparison …
