(A) Heatmaps showing ChIP-seq enrichment for the DCC recruiter proteins, SDC-2, SDC-3, and DPY-30, the condensin DC subunit DPY-27, and the histone modification H3K4me3. Heatmaps are plotted across a 3 kb window centered on the 64 recruitment sites. Based on the DCC ChIP enrichment scores, the recruitment sites are categorized into three strength classes (strong, intermediate, weak) using k-means clustering (n = 3). (B) The strong DCC recruitment sites (pink, n = 17) are scattered across the X chromosome. Intermediate (n = 16) and weak (n = 31) recruitment sites (gray) are distributed in between. The median distance between recruitment sites is ~90 kb. SDC-2 enrichment across the length of the X chromosome was plotted using IGV. (C) In an sdc-3 null mutant, SDC-2 binds the strongest recruitment sites, indicating hierarchy of recruitment. As in (A), heatmaps indicating SDC-2 ChIP-seq enrichment are plotted across a 3 kb window centered on the 64 recruitment sites. Data from wild-type, sdc-3 null, and sdc-2 null are all plotted on the same scale. Density plots above each heatmap indicate the average ChIP enrichment score for each recruitment class plotted across the 3 kb window. Strong sites are plotted in pink, intermediate in blue, and weak in grey.