DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids
The number of children born since the origin of Assisted Reproductive Technologies (ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has been reported among ART-conceived infants, suggesting an epigenetic cost. We report the first whole-genome DNA methylation datasets from single pig blastocysts showing differences between in vivo and in vitro produced embryos. Blastocysts were produced in vitro either without (C-IVF) or in the presence of natural reproductive fluids (Natur-IVF). Natur-IVF embryos were of higher quality than C-IVF in terms of cell number and hatching ability to. RNA-Seq and DNA methylation analyses showed that Natur-IVF embryos have expression and methylation patterns closer to in vivo blastocysts. Genes involved in reprogramming, imprinting and development were affected by culture, with fewer aberrations in Natur-IVF embryos. Methylation analysis detected methylated changes in C-IVF, but not in Natur-IVF, at genes whose methylation could be critical, such as IGF2R and NNAT.
Data from: DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluidsAvailable at Dryad Digital Repository under a CC0 Public Domain Dedication.
Article and author information
Research Councils UK
- Gavin Kelsey
Ministerio de Economía y Competitividad (AGL2012-40180-C03-01 and AGL2015-66341-R)
- Pilar Coy
Ministerio de Educación, Cultura y Deporte (PRX14/00348)
- Pilar Coy
Fundación Séneca. Región de Murcia. Spain (20040/GERM/16)
- Pilar Coy
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Animal experimentation: This study was carried out in strict accordance with the recommendations in the Guiding Principles for the Care and Use of Animals (DHEW Publication, NIH, 80-23). The protocol was approved by the Ethical Committee for Experimentation with Animals of the University of Murcia, Spain (Project Code: 192/2015).
- Jessica K Tyler, Weill Cornell Medicine, United States
- Received: November 26, 2016
- Accepted: January 28, 2017
- Accepted Manuscript published: January 30, 2017 (version 1)
- Accepted Manuscript updated: February 1, 2017 (version 2)
- Version of Record published: March 7, 2017 (version 3)
- Version of Record updated: April 11, 2017 (version 4)
© 2017, Canovas et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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