(A) The full Cdc3 sequence was analyzed by DISOPRED3 for predicted disorder. Plots show confidence scores for predicted disorder for each residue. The shaded area of the plot at left is expanded in the plot at right. (B) Sequence alignment of the first 97 amino acids of S. cerevisiae Cdc3 (ScCdc3) with Cdc3 homologs from other fungal species that have an obvious NTE. Average DISOPRED3 scores for each NTE (residues Nterminal to the conserved motif) are given in the column labeled ‘Diso’. The percentage value at far right gives the percent identity compared to ScCdc3 residues 88–520. Blue and red indicate similar and identical residues, respectively. Species and accession numbers: Sk, S. kudriavzevii, EJT44570.1; Nc, Naumovozyma castellii, XP_003675929.1; Zb, Zygosaccharomyces bailii, CDF90019.1; Td, Torulaspora delbrueckii, XP_003682181.1; Ag, Ashbya gossypii, NP_985658.2; Cg,Candida glabrata, KTA97432.1; Lm, Lachancea mirantina, SCV04072.1; Km, Kluyveromyces marxianus, BAO38302.1; Lq, Lachancea quebecensis, CUS21187.1; Kl, Kluyveromyces lactis XP_453247.1; Ho, Hanseniaspora osmophila, OEJ91001.1. (C) As in (B), but with fungal species that lack an NTE. Consequently, no DISOPRED3 score is given. Species and accession numbers: Mb, Metschnikowia bicuspidata, XP_018712407.1; Cm, Candida maltosa, EMG46918.1; Ct, C. tropicalis, XP_002550438.1; Ca, C. albicans, P39826.1; Pm, Pichia membranifaciens, XP_019015360.1; Ta, Tilletiaria anomala, XP_013243186.1. (D) The remainder of the ScCdc3 protein sequence is given with the color scheme as in (B) showing similarity and identity with the species in (B). Notable structural features are indicated above the sequence.