(A) Phylogenetic tree including strains of Streptomyces bacteria and Bacillus subtilis. (B) Schematic diagram of a dry-drop behavioral assay that selectively tests the response of the PHA and PHB …
Source data for Figure 1 and Figure 1—figure supplement 1.
Significance for pairwise comparisons for Figure 1.
(A) Chemotaxis assays are designed to test olfactory cues (Bargmann et al., 1993). Chemotaxis plates included an origin, where the nematodes are placed, and control and experimental sections. Both …
(A) Quantification of the similar phasmid responses to 3.4 mM and 0.6 mM SDS, which is ten-fold below the critical micelle concentration of SDS. All other assays in this study were performed with …
Source data for Figure 2 and Figure 2—figure supplement 2.
Significance for pairwise comparisons for Figure 2 and Figure 2—figure supplement 2.
The response to SDS, dodecanoate and decanoate at the tail is shown. Note that compounds are represented as the predominant form in a pH 7 solution, the carboxylates. One-way ANOVA and Tukey’s …
Carboxylates were extracted from species of Streptomyces and labeled with a pyridinium moiety for easier detection by mass spectrometry. For example, to detect dodecanoate after Streptomyces …
Modified decanoic (C10) and dodecanoic (C12) acids were detected by LC-MS by searching for molecular weights of 278 and 306 g/mol, respectively. Modified decanoic (C10) and dodecanoic (C12) acid …
(A) Quantification of the defect in the phasmid response to SDS, Streptomyces, dodecanoate and decanoate in srb-6 null mutants. (B) Quantification of the defect in the amphid response to Streptomyces…
Source data for Figure 4 and Figure 4—figure supplement 1.
Significance for pairwise comparisons for Figure 4—figure supplement 1.
RNAi was used to knock down the expression of chemoreceptor genes (A) expressed in PHA, PHB, and a subset of neurons in the head and (B) srb family members. (A-B) RNAi was performed in an eri-1(mg366…
srb-6 is highlighted in red. Note that srb-6’s closest homologs (highlighted in dark green) are within the Caenhorabditis genus, and a larger monophyletic group contains nematode species outside the …