Autophagy functions as an antiviral mechanism against geminiviruses in plants
- School of Life Sciences, Tsinghua University, China
- Hangzhou Normal University, China
Abstract
Autophagy is an evolutionarily conserved process that recycles damaged or unwanted cellular components, and has been linked to plant immunity. However, how autophagy contributes to plant immunity is unknown. Here we reported that the plant autophagic machinery targets the virulence factor βC1 of Cotton leaf curl Multan virus (CLCuMuV) for degradation through its interaction with the key autophagy protein ATG8. A V32A mutation in βC1 abolished its interaction with NbATG8f, and virus carrying βC1V32A showed increased symptoms and viral DNA accumulation in plants. Furthermore, silencing of autophagy-related genes ATG5 and ATG7 reduced plant resistance to the DNA viruses CLCuMuV, Tomato yellow leaf curl virus, and Tomato yellow leaf curl China virus, whereas activating autophagy by silencing GAPC genes enhanced plant resistance to viral infection. Thus, autophagy represents a novel anti-pathogenic mechanism that plays an important role in antiviral immunity in plants.
Article and author information
Author details
Funding
National Natural Science Foundation of China (31530059)
- Yule Liu
National Natural Science Foundation of China (31421001)
- Yule Liu
National Natural Science Foundation of China (31470254)
- Yule Liu
National Natural Science Foundation of China (31370180)
- Yule Liu
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Jian-Min Zhou, Chinese Academy of Sciences, China
Version history
- Received: December 6, 2016
- Accepted: February 26, 2017
- Accepted Manuscript published: February 28, 2017 (version 1)
- Accepted Manuscript updated: March 2, 2017 (version 2)
- Version of Record published: March 22, 2017 (version 3)
Copyright
© 2017, Haxim et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Autophagy functions as an antiviral mechanism against geminiviruses in plants
eLife 6:e23897.
https://doi.org/10.7554/eLife.23897
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