(A) Unrooted phylogenetic tree of the Arabidopsis 14-3-3 protein family. Maximum parsimony analyses were performed using PAUP 4.0b10 (Altivec) with the bootstrap-algorithm and 1000 replica. (B, C) Expression of iota-GFP is restricted to pollen. (D) to (G) Pi-GFP is exclusively expressed in the chalazal cyst of the seed (D, E) and the connective tissue of the anthers (F, G). (H) to (O) The expression of nu-GFP is ubiquitous. Expression in seeds (H), seedlings (I), root tips (J), roots (K), hypocotyl (L), leaf epidermis (M) and floral organs (N, O) is shown. (P) to (S) Mu-GFP (P, R) and merge with FM4-64 (Q, S) in epidermal root cells (elongation zone) of seedlings (4d) treated with CHX (50 μM) for 60 min followed by treatment with CHX (P, Q) or CHX and 50 μM BFA (R, S) for 60 min. Note that mu-GFP localizes to both nucleus and cytoplasm but accumulates in BFA bodies upon inhibitor treatment. Arrowheads indicate nuclei, arrows mark BFA bodies.