Palaeogenomes of Eurasian straight-tusked elephants challenge the current view of elephant evolution

  1. Matthias Meyer  Is a corresponding author
  2. Eleftheria Palkopoulou
  3. Sina Baleka
  4. Mathias Stiller
  5. Kirsty E H Penkman
  6. Kurt W Alt
  7. Yasuko Ishida
  8. Dietrich Mania
  9. Swapan Mallick
  10. Tom Meijer
  11. Harald Meller
  12. Sarah Nagel
  13. Birgit Nickel
  14. Sven Ostritz
  15. Nadin Rohland
  16. Karol Schauer
  17. Tim Schüler
  18. Alfred L Roca
  19. David Reich
  20. Beth Shapiro
  21. Michael Hofreiter  Is a corresponding author
  1. Max Planck Institute for Evolutionary Anthropolgy, Germany
  2. Harvard Medical School, United States
  3. University of Potsdam, Germany
  4. University of York, United Kingdom
  5. Danube Private University, Austria
  6. Basel University, Switzerland
  7. University of Illinois at Urbana-Champaign, United States
  8. State Office for Heritage Management and Archaeology Saxony-Anhalt with State Museum of Prehistory, Germany
  9. Naturalis Biodiversity Center, Netherlands
  10. Thüringisches Landesamt für Denkmalpflege und Archäologie, Germany
  11. Broad Institute of Harvard and MIT, United States
  12. Howard Hughes Medical Institute, Harvard Medical School, United States
  13. University of California, United States
3 figures

Figures

Palaeoloxodon antiquus, geographic range based on fossil finds (after Pushkina, 2007).

White dots indicate the locations of Weimar-Ehringsdorf and Neumark-Nord.

https://doi.org/10.7554/eLife.25413.003
Figure 2 with 5 supplements
Phylogenetic trees relating the mitochondrial and nuclear sequences of P.antiquus (NN and WE) to other elephantids.

(A) Maximum clade credibility (MCC) tree resulting from a BEAST (Drummond et al., 2012) analysis of 35 complete mitochondrial genomes using 15,447 sites. Node bars and numbers show the 95% highest …

https://doi.org/10.7554/eLife.25413.004
Figure 2—source data 1

This spreadsheet contains summary statistics of all sequence data generated in this study, the sequences of PCR primers used for reconstructing mtDNA sequences of extant elephants, as well as amino acid racemization data on opercula of Bithynia tentaculata from Amersfoort.

https://doi.org/10.7554/eLife.25413.005
Figure 2—figure supplement 1
Sequence coverage of the NN and WE mitochondrial genomes.
https://doi.org/10.7554/eLife.25413.006
Figure 2—figure supplement 2
DNA fragment size distribution inferred from full-length mtDNA sequences.
https://doi.org/10.7554/eLife.25413.007
Figure 2—figure supplement 3
Frequency of C to T substitutions for each position in the sequence alignments.

(A) Substitution frequencies in mitochondrial alignments. Substitution frequencies are depressed in the Neumark-Nord libraries due treatment with uracil-DNA-glycosylase (UDG). (B) In nuclear …

https://doi.org/10.7554/eLife.25413.008
Figure 2—figure supplement 4
Maximum likelihood tree from concatenated nuclear protein-coding sequences with bootstrap support values shown inside nodes.
https://doi.org/10.7554/eLife.25413.009
Figure 2—figure supplement 5
Amino acid racemization data.

D/L values of Asx, Glx, Ala and Val for the free amino acid (FAA, panels on the left) and total hydrolysable amino acid (THAA, panels on the right) fraction of bleached Bithynia tentaculata opercula …

https://doi.org/10.7554/eLife.25413.010
A revised tree of phylogenetic relationships among elephantids, color-coded by their presumed geographical range.
https://doi.org/10.7554/eLife.25413.011

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