(A) Log scores of RPM of 171 alleles detected in tissue-enriched samples and hands from four quaternary limbs. Each column represents one allele across 6 tissue-enriched samples and hand. Alleles found at a high frequency in the hands of animals (most rightward 15 columns) are present at high frequency across most tissue samples (mean = 5.4 of 6 tissues, s.d. =±1.1, n = 15/171 alleles,). Tissue-enriched alleles are collected among alleles that are low-frequency in the hand (57/58; 27/58 not detected). (B) Pie chart depicting tissue-enriched alleles across tissues. Tissue-enriched alleles overrepresented in two tissue types are categorized by the single tissue in which they are most represented. (C) Heat map depicting P values of Fisher’s Exact tests comparing observed and expected distributions of tissue-enriched alleles among those that changed in frequency from primary to secondary, secondary to tertiary, and primary to tertiary limbs. Data in Figure 5—source data 1.