gene (Danio rerio) | npvf | PMID: 26687719 | ZDB-GENE- 070424–226 | |
gene (Caenorhabditis elegans) | flp-13 | PMID: 19910365 | CELE_F33D4.3; WBGene00001456 | |
genetic reagent (D. rerio) | Tg(hs:NPVF) | this paper | ct846Tg; RRID: ZDB-ALT-170927-2 | zebrafish npvf open reading frame cloned downstream of the zebrafish hsp70c promoter |
genetic reagent (D. rerio) | Tg(hs:RFRP1) | this paper | ct854Tg; RRID: ZDB-ALT-171004-1 | NPVF transgene cloned downstream of the zebrafish hsp70c promoter, but with the amino acid sequence of RFRP2 and RFRP3 scrambled to abolish peptide function |
genetic reagent (D. rerio) | Tg(hsp:RFRP2) | this paper | ct855Tg; RRID: ZDB-ALT-171004-2 | NPVF transgene cloned downstream of the zebrafish hsp70c promoter, but with the amino acid sequence of RFRP1 and RFRP3 scrambled to abolish peptide function |
genetic reagent (D. rerio) | Tg(hs:RFRP3) | this paper | ct856Tg; RRID: ZDB-ALT-171004-3 | NPVF transgene cloned downstream of the zebrafish hsp70c promoter, but with the amino acid sequence of RFRP1 and RFRP2 scrambled to abolish peptide function |
genetic reagent (D. rerio) | Tg(hs:RFRP1,2) | this paper | ct857Tg; RRID: ZDB-ALT-171004-4 | NPVF transgene cloned downstream of the zebrafish hsp70c promoter, but with the amino acid sequence of RFRP3 scrambled to abolish peptide function |
genetic reagent (D. rerio) | Tg(hs:RFRP1,3) | this paper | ct858Tg; RRID: ZDB-ALT-171004-5 | NPVF transgene cloned downstream of the zebrafish hsp70c promoter, but with the amino acid sequence of RFRP2 scrambled to abolish peptide function |
genetic reagent (D. rerio) | Tg(hs:RFRP2,3) | this paper | ct859Tg; RRID: ZDB-ALT-171004-6 | NPVF transgene cloned downstream of the zebrafish hsp70c promoter, but with the amino acid sequence of RFRP1 scrambled to abolish peptide function |
genetic reagent (D. rerio) | Tg(hs:RFRPscr) | this paper | ct860Tg; RRID: ZDB-ALT-171004-7 | NPVF transgene cloned downstream of the zebrafish hsp70c promoter, but with the amino acid sequence of RFRP1, RFRP2, and RFRP3 scrambled to abolish peptide function |
genetic reagent (D. rerio) | Tg(hs:FLP-13) | this paper | | C. elegans flp-13 open reading frame cloned downstream of the zebrafish hsp70c promoter |
genetic reagent (D. rerio) | npvf mutant | this paper | ct845; RRID: ZDB-ALT-170927-1 | mutant contains a 7 bp deletion after nucleotide 324 of the open reading frame; premature stop codon after amino acid 146 |
genetic reagent (D. rerio) | Tg(npvf:EGFP) | this paper | ct847Tg; RRID: ZDB-ALT-170927-3 | npvf promoter cloned upstream of enhanced green fluorescent protein (EGFP) |
genetic reagent (D. rerio) | Tg(npvf:KalTA4) | this paper | ct848Tg; RRID: ZDB-ALT-170927-4 | npvf promoter cloned upstream of KalTA4 |
genetic reagent (D. rerio) | Tg(npvf:ReaChR- mCitrine) | this paper | ct849Tg; RRID: ZDB-ALT-170927-5 | npvf promoter cloned upstream of ReaChR-mCitrine |
genetic reagent (D. rerio) | Tg(npvf:mTagYFP- T2A-eNTR) | this paper | ct850Tg; RRID: ZDB-ALT-170927-6 | npvf promoter cloned upstream of mTagYFP-T2A- enhanced nitroreductase |
genetic reagent (D. rerio) | Tg(UAS:TRPV1- TagRFP-T; cmlc2:EGFP) | this paper | ct851Tg; RRID: ZDB-ALT-170927-7 | UAS promoter cloned upstream of TRPV1-TagRFP-T |
genetic reagent (D. rerio) | Tg(elavl3:H2B- GCaMP6s) | PMID: 25068735 | jf5Tg; RRID: ZDB-ALT-141023-2 | |
antibody | anti-GFP (Chicken polyclonal) | Aves Laboratories | Cat#: GFP-1020; RRID: AB_10000240 | 1:400 |
antibody | anti-TagRFP (Rabbit polyclonal) | Evrogen | Cat#: AB233 RRID: AB_2571743 | 1:100 |
antibody | anti-dsRed (Rabbit polyclonal) | Takara Clonetech | Cat#: 645496 | 1:400 |
antibody | anti-digoxigenin Fab fragments (Sheep polyclonal) | Sigma-Aldrich | Cat#: 11093274910; RRID: AB_514497 | 1:2000 |
antibody | Alexa 488- or 568- secondaries | Molecular Probes | | 1:500 |
sequence-based reagent | Primers for genotyping | this paper | | See Materials and Methods |
sequence-based reagent | Primers for qPCR | this paper | | See Materials and Methods |
sequence-based reagent | Primers for riboprobe synthesis | this paper | | See Materials and Methods |
sequence-based reagent | Oligonucleotides used to generate npvf CRISPR mutant | this paper | | See Materials and Methods |
peptide, recombinant protein | RF9 | Tocris | Cat #: 3672 | |
peptide, recombinant protein | GJ-14 | PMID: 26259035 | Anaspec, Inc; custom synthesis | |
peptide, recombinant protein | Normal Goat Serum | Thermo Fisher Scientific | Cat# NC9660079 | |
commercial assay or kit | DIG RNA Labeling Kit | Sigma-Aldrich | Cat#: 11175025910 | |
commercial assay or kit | TSA Plus Cyanine 3 and Fluorescein System | PerkinElmer | Cat#: NEL753001KT | |
commercial assay or kit | FirstChoice RLM-RACE | Thermo Fisher Scientific | Cat#: AM1700 | |
commercial assay or kit | SuperScript III First-Strand Synthesis System | Thermo Fisher Scientific | Cat#: 18080051 | |
chemical compound, drug | 16% paraformaldehyde | Thermo Fisher Scientific | Cat#: 15710 | |
chemical compound, drug | NBT/BCIP Stock Solution | Thermo Fisher Scientific | Cat#: 11681451001 | |
chemical compound, drug | SYBR Green PCR Master Mix | Thermo Fisher Scientific | Cat#: 4364346 | |
software, algorithm | Excel | Microsoft | https://office.microsoft.com/excel/ | |
software, algorithm | Fiji | PMID: 22732772 | https://fiji.sc; RRID: SCR_002285 | |
software, algorithm | GraphPad Prism6 | GraphPad Software | http://www.graphpad.com/; RRID: SCR_002798 | |
software, algorithm | MATLAB | MathWorks | https://www.mathworks.com/ | |
software, algorithm | Principal Component and Independent Component Analysis | PMID: 19778505 | | Automated analysis of cellular signals from large-scale calcium imaging data. |
software, algorithm | Videotracking script for behavioral analysis | PMID: 17182791 | | Source code attached |
software, algorithm | Arousal threshold script for behavioral analysis | PMID: 26374985 | | Source code attached |
other | 96-well plate for behavioral experiments | GE Healthcare Life Sciences | Cat#: 7701–1651 | |
other | MicroAmp Optical Adhesive Film | Thermo Fisher Scientific | Cat#: 4311971 | |