Soft X-ray tomography shows loss of mechanical integrity of the red cell membrane in the final stages of egress. Panels A-C depict schizonts treated with a selective malarial cGMP-dependent protein kinase G inhibitor (C2), and panels D-F depict schizonts treated with a broad-spectrum cysteine protease inhibitor, E64, which allows parasitophorous vacuole membrane (PVM) rupture but prevents erythrocyte membrane rupture, resulting in merozoites trapped in the blood cell. (A) Slice from tomogram of C2-arrested schizont. (B) Outlines of erythrocyte membrane (red), PVM (yellow), and parasites (cyan) in the tomogram slice in A. (C) 3D rendering of the schizont. The vacuole (yellow) is densely packed with merozoites (cyan) that have been collectively rather than individually rendered, for clarity. The overall height of the cell is ∼5 μm. (D) Tomogram slice from an E64-arrested schizont, shown with outlining of membranes in E. Remnants of the PVM are visible. (F) 3D rendering of the schizont. Figure and legend adapted with permission from Hale et al. (2017). Scale bar 1 μm.