Eyeless uncouples mushroom body neuroblast proliferation from dietary amino acids in Drosophila
Abstract
Cell proliferation is coupled with nutrient availability. If nutrients become limited, proliferation ceases, because growth factor and/or PI3-kinase activity levels become attenuated. Here, we report an exception to this generality within a subpopulation of Drosophila neural stem cells (neuroblasts). We find that most neuroblasts enter and exit cell cycle in a nutrient-dependent manner that is reversible and regulated by PI3-kinase. However, a small subset, the mushroom body neuroblasts, which generate neurons important for memory and learning, divide independent of dietary nutrient conditions and PI3-kinase activity. This nutrient-independent proliferation is regulated by Eyeless, a Pax-6 orthologue, expressed in mushroom body neuroblasts. When Eyeless is knocked down, mushroom body neuroblasts exit cell cycle when nutrients are withdrawn. Conversely, when Eyeless is ectopically expressed, some non-mushroom body neuroblasts divide independent of dietary nutrient conditions. Therefore, Eyeless uncouples MB neuroblast proliferation from nutrient availability, allowing preferential neurogenesis in brain subregions in nutrient poor environments.
Article and author information
Author details
Funding
NIH Office of the Director (R00-HD067293)
- Conor W Sipe
- Sarah E Siegrist
NIH Office of the Director (R01-GM120421)
- Conor W Sipe
- Sarah E Siegrist
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Bruce Edgar, University of Utah, United States
Version history
- Received: February 24, 2017
- Accepted: August 8, 2017
- Accepted Manuscript published: August 9, 2017 (version 1)
- Version of Record published: August 24, 2017 (version 2)
- Version of Record updated: January 4, 2019 (version 3)
Copyright
© 2017, Sipe & Siegrist
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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