(A) Plots of the maximal surface pressure variation (ΔΠ) vs. the initial surface pressure (Πi) and of the corresponding maximal insertion pressure (MIP) obtained from the adsorption experiments with Langmuir through for REM-CA peptide, with different monolayer compositions i.e. PC alone, PC:PIPs (molar ratio 80:20) or PC:PIPs:Sito (molar ratio 70:20:10). (B) Fourier Transformed-InfraRed (FT-IR) spectra of REM-CA, R1 and R2 peptides in presence of different composition of MLV i.e. PLPC alone, PC:PIP (molar ratio 80:20), PC:Sito (molar ratio 90:10) or PC:PIP:Sito (molar ratio 70:20:10). Three regions of interest of the spectra are shown: the 3050–2800 cm−1 region corresponds to the absorbance of lipid alkyl chains, the 1295–1125 cm−1 region to the lipid phosphate groups and the 1800–1600 cm−1 region to the lipid C=O ester and peptide amide groups. (C) Solid-state NMR structure of REM-CA peptides 13C-labeled at the positions L180, G188 and I184. NMR spectra are shown for membranes containing REM-CA peptides, composed of deuterated 1,2-dimyristoyl-D54-sn-3-phosphocholine, (DMPC-d54), supplemented with sitosterol and Phosphoinositides mix (PIPs), see Figure 2—figure supplement 1B,C.