Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of Drosophila

  1. Yongjun Qian
  2. Yue Cao
  3. Bowen Deng
  4. Guang Yang
  5. Jiayun Li
  6. Rui Xu
  7. Dandan zhang
  8. Juan Huang  Is a corresponding author
  9. Yi Rao  Is a corresponding author
  1. Peking University, China
  2. Cold Spring Harbor Laboratory, United States
  3. Nanjing Medical University, China

Abstract

Our understanding of molecular mechanisms underlying sleep homeostasis is limited. We have taken a systematic approach to study neural signaling by the transmitter 5-hydroxytryptamine (5-HT) in Drosophila. We have generated knockout and knockin lines for Trh, the 5-HT synthesizing enzyme and all five 5-HT receptors, making it possible for us to determine their expression patterns and to investigate their functional roles. Loss of the Trh, 5HT1a or 5HT2b gene decreased sleep time whereas loss of the Trh or 5HT2b gene diminished sleep rebound after sleep deprivation. 5HT2b expression in a small subset of neurons in the dorsal fan-shaped body (dFB) is functionally essential: elimination of the 5HT2b gene from these neurons led to loss of sleep homeostasis. Genetic ablation of 5HT2b neurons in the dFB decreased sleep and impaired sleep homeostasis. Our results have shown that serotonergic signaling in specific neurons is required for regulating sleep homeostasis.

Article and author information

Author details

  1. Yongjun Qian

    Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
  2. Yue Cao

    Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
  3. Bowen Deng

    Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
  4. Guang Yang

    Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
    Competing interests
    The authors declare that no competing interests exist.
  5. Jiayun Li

    Cold Spring Harbor Laboratory, Cold Spring Harbor, United States
    Competing interests
    The authors declare that no competing interests exist.
  6. Rui Xu

    School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China
    Competing interests
    The authors declare that no competing interests exist.
  7. Dandan zhang

    School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China
    Competing interests
    The authors declare that no competing interests exist.
  8. Juan Huang

    School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China
    For correspondence
    huangjuan@njmu.edu.cn
    Competing interests
    The authors declare that no competing interests exist.
  9. Yi Rao

    Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
    For correspondence
    yrao@pku.edu.cn
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-0405-5426

Funding

National Natural Science Foundation of China (Project 31421003)

  • Yi Rao

National Natural Science Foundation of China (Project 31000547)

  • Juan Huang

Beijing Municipal Natural Science Foundation (Z111107067311058)

  • Yi Rao

Beijing Municpal Natural Science Foundation (Z151100003915121)

  • Yi Rao

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Amita Sehgal, Howard Hughes Medical Institute, University of Pennsylvania, United States

Publication history

  1. Received: March 7, 2017
  2. Accepted: October 5, 2017
  3. Accepted Manuscript published: October 6, 2017 (version 1)
  4. Version of Record published: October 19, 2017 (version 2)

Copyright

© 2017, Qian et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Yongjun Qian
  2. Yue Cao
  3. Bowen Deng
  4. Guang Yang
  5. Jiayun Li
  6. Rui Xu
  7. Dandan zhang
  8. Juan Huang
  9. Yi Rao
(2017)
Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of Drosophila
eLife 6:e26519.
https://doi.org/10.7554/eLife.26519

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