Percentage of cells scored as mesenchyme in freshly dissected tips and stalks. Expressed as percentage of non-ECAD+ cells from total DAPI+ cells. Quantitation done using the mesenchyme-macro.ijm for FIJI which is available as a supplemental file.
Percentage organoid forming efficiency and size increase from D1-11 of culture when fresh tips are plated in self-renewing medium +/- SB43125. Three independent experiments were performed on different lung samples.
Transcription Factors with mouse orthologues upregulated > 2 fold in human tips or stalks: evidence for mouse expression pattern (related to Figure 2J and L).