(A) Plants homozygously expressing ATeam in the cytosol (cyt), plastids (cp) or mitochondria (mt) were grown vertically for 5 days on solidified half-strength MS medium side by side with wild-type Col-0. Fluorescence of plant material was checked with an epifluorescence microscope equipped with a GFP filter. Primary root length of 35 ATeam and 35 Col-0 plants per independent sensor line was quantified 5 days after stratification and statistical difference from Col-0 was tested with a one-way ANOVA followed by the Dunnett test. ns: p>0.05, ***p≤0.001; error bars = SD. (B) 20 (Col-0, cyt, cp) and 12 (mt) randomly selected plants shown in (A) were transferred to soil and grown in long-day growth chambers. Rosette development was documented photographically and the leaf rosette area was analysed until the majority of plants developed first open flowers. Growth curves for each sensor line are plotted against the same set of Col-0 plants and statistical differences were assessed for individual timepoints separately with a one-way ANOVA, followed by the Dunnett test. *p≤0.05, ***p≤0.001; error bars = SD. (C) Primary inflorescence height of 46-d-old plants was captured photographically and quantified. Siliques of 61-d-old plants were manually counted. n = 20; error bars = SD; ns: p>0.05 (one-way ANOVA with Dunnett test to compare sensor lines with Col-0). Scale bar = 50 mm. Plants expressing mitochondrial ATeam did not bolt until day 61 and were therefore not included in the analysis.