(A) RNA prepared from embryos at 5 dpf of the indicated genotypes were subjected to RNA-seq analysis and the data obtained are expressed as MA plot. Genes whose expression was significantly altered in IRE1α/β-DKO compared with IRE1β-KO are colored and summarized in the table below. Grey dots denote genes without GO. n = 4 for IRE1β-KO and n = 3 for IRE1α/β-DKO XBP1+/+ and IRE1α/β-DKO XBP1SC/+. (B) 200 significantly up-regulated and 26 significantly down-regulated genes are categorized based on their assigned GO. GO:0006986, GO:0030968, GO:0034620, GO:0034966, GO:0034976 and GO:0035967 for UPR (1); GO:0006457, GO:0016485 and GO:0051604 for Folding (2); GO:0030433, GO:0030970 and GO:0036503 for ERAD (3); GO:0006486, GO:0006487, GO:0009100, GO:0009101, GO:0018279, GO:0043413 and GO:0070085 for Glycosylation (4); GO:0006888, GO:0006890, GO:0006900, GO:0006901, GO:0006903, GO:0048193, GO:0048199, GO:0048207, GO:0048208 and GO:0090114 for Protein transport (5); GO:0006612, GO:0006613, GO:0006614, GO:0045047, GO:0070972, GO:0072594, GO:0072599, GO:0072657 and GO:0090150 for Protein targeting to ER (6); GO:0051640 and GO:0051656 for Organelle localization (7); GO:0006914 for Autophagy (8); GO:0006644, GO:0008610 and GO:0046486 for Lipid biosynthetic process (9); GO:0005975 for Carbohydrate metabolic process (10); GO:0006164 and GO:0009117 for Nucleotide metabolic process (11); GO:0006094 for Gluconeogenesis (12); GO:0055076 for Transition metal ion homeostasis (13); GO:0006873 for Cellular ion homeostasis (14); GO:0007601 for Visual perception (15); GO:0007517 and GO:0090257 for Muscle tissue development (16); GO:0051216 for Cartilage development (17); GO:0042391 for Regulation of membrane potential (18); GO:0031099 for Regeneration (19).