A connectome of a learning and memory center in the adult Drosophila brain
- Janelia Research Campus, Howard Hughes Medical Institute, United States
Abstract
Understanding memory formation, storage and retrieval requires knowledge of the underlying neuronal circuits. In Drosophila, the mushroom body (MB) is the major site of associative learning. We reconstructed the morphologies and synaptic connections of all 983 neurons within the three functional units, or compartments, that compose the adult MB’s α lobe, using a dataset of isotropic 8-nm voxels collected by focused ion-beam milling scanning electron microscopy. We found that Kenyon cells (KCs), whose sparse activity encodes sensory information, each make multiple en passant synapses to MB output neurons (MBONs) in each compartment. Some MBONs have inputs from all KCs, while others differentially sample sensory modalities. Only six percent of KC>MBON synapses receive a direct synapse from a dopaminergic neuron (DAN). We identified two unanticipated classes of synapses, KC>DAN and DAN>MBON. DAN activation produces a slow depolarization of the MBON in these DAN>MBON synapses and can weaken memory recall.
Article and author information
Author details
Funding
Howard Hughes Medical Institute
- Harald F Hess
- Glenn C Turner
- Gerald M Rubin
- Louis K Scheffer
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Ronald L Calabrese, Emory University, United States
Version history
- Received: March 19, 2017
- Accepted: July 17, 2017
- Accepted Manuscript published: July 18, 2017 (version 1)
- Accepted Manuscript updated: July 19, 2017 (version 2)
- Version of Record published: August 9, 2017 (version 3)
Copyright
© 2017, Takemura et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A connectome of a learning and memory center in the adult Drosophila brain
eLife 6:e26975.
https://doi.org/10.7554/eLife.26975
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